组氨酸对DNA电荷逆转和凝聚的影响

doi:10.3976/j.issn.1002-4026.2018.04.019

山东科学 ›› 2018, Vol. 31 ›› Issue (4): 126-132.doi: 10.3976/j.issn.1002-4026.2018.04.019

组氨酸对DNA电荷逆转和凝聚的影响

芦洁坪，王艳伟，杨光参*

温州大学数理与电子信息工程学院，浙江温州 325035

收稿日期:2018-04-03 出版日期:2018-08-20 发布日期:2018-08-20
通信作者: 杨光参。E-mail:yanggc@wzu.edu.cn E-mail:yanggc@wzu.edu.cn
作者简介:芦洁坪（1993—），女，硕士研究生，研究方向为生命现象中的凝聚态物理。
基金资助:
国家自然科学基金（11574232，11274245,11304232）

Histidine induced DNA charge inversion and condensation

LU Jie-ping, WANG Yan-wei, YANG Guang-can*

School of Math and Electronic Information Engineering, Wenzhou University, Wenzhou 325035, China

Received:2018-04-03 Online:2018-08-20 Published:2018-08-20

摘要/Abstract

摘要：

以组氨酸为研究对象，通过动态光散射观察到当组氨酸浓度为1 mg/mL时，电泳迁移率由负变为正，DNA发生电荷逆转。同时通过原子力显微镜可以观察到，随着组氨酸浓度的升高，DNA分子逐渐堆积折叠，直至在组氨酸浓度为1 mg/mL时凝聚成球，说明了组氨酸可以导致DNA凝聚和电荷逆转。由于组氨酸的等电位点为7.59，相信组氨酸侧链的质子化和去质子化对其带电状态起调节作用。作为生物体内的基本遗传物质，DNA影响着生物的生长发育，与细胞癌变、突变等异常活动也息息相关。在细胞中，组蛋白是DNA染色体的重要组成部分，在DNA的转录和复制等过程中调节DNA的凝聚状态。组氨酸是组蛋白的主要成分，研究组氨酸对DNA凝聚以及相关的电荷逆转过程的影响，对于理解组蛋白的调控作用有重要的基础意义。

关键词: 组氨酸, 电荷逆转, 凝聚, DNA

Abstract:

Taking histidine as the research object, it was observed by dynamic light scattering that the electrophoretic mobility changes from negative to positive, and reversal of DNA charge when the histidine concentration was 1 mg/mL. At the same time, the following phenomena could be observed by atomic force microscopy: as the concentration of histidine increased, DNA molecules gradually piled up and folded up to a sphere at a histidine concentration of 1 mg/mL, which indicated that histidine could lead to DNA condensation and charge reversal. Since the isoelectric point of histidine is 7.59, it is believed that the protonation and deprotonation of the histidine side chain play a role in the regulation of its charged state. As a basic genetic material in the organism, DNA affects the growth and development of organisms, which is also closely related to abnormal activities such as cell cancer and mutation. In cells, histones are significant part of the DNA chromosomes and regulate DNA condensation in the process of DNA transcription and replication. Histidine is the main component of histones. The study of the effect of histidine on DNA condensation and related charge reversal processes is of fundamental importance for understanding the regulation of histones.

Key words: histidine, condensation, DNA, charge inversion

中图分类号:

芦洁坪，王艳伟，杨光参. 组氨酸对DNA电荷逆转和凝聚的影响[J]. 山东科学, 2018, 31(4): 126-132.

LU Jie-ping, WANG Yan-wei, YANG Guang-can. Histidine induced DNA charge inversion and condensation[J]. SHANDONG SCIENCE, 2018, 31(4): 126-132.

参考文献

［1］AMADUZZI F, BOMBOI F, BONINCONTRO A, et al. ChitosanDNA complexes: charge inversion and DNA condensation[J]. Colloids and Surfaces B: Biointerfaces. 2014, 114:110.
[2]MANNING G S. The molecular theory of polyelectrolyte solutions with applications to the electrostatic properties of polynucleotides[J]. Quarterly Reviews of Biophysics, 1978, 11(2):179246.
[3]SIVASANKAR S, SUBRAMANIAM S, LECKBAND D. Direct molecular level measurements of the electrostatic properties of a protein surface [J]. Proceedings of the National Academy of Sciences of the United States of America, 1998, 95(22):12961 12966.
[4]PODGORNIK R. Liposomes in gene delivery by Danilo D. lasic [J]. Biophysical Journal, 1998,74(4):21382139.
[5]MANNING G S. Counterion binding in polyelectrolyte theory [J]. Accounts of Chemical Research, 1979, 12(12):443449.
[6]WONG G C, POLLACK L. Electrostatics of strongly charged biological polymers:Ionmediated interactions and selforganization in nucleic acids and proteins [J]. Annual Review of Physical Chemistry, 2010, 61:171189.

[7]GROSBERG A Y, NGUYEN T T, SHKLOVSKII B I. Colloquium:The physics of charge inversion in chemical and biological systems [J]. Reviews of modern physics, 2002, 74(2):329345.
[8]QIU S X, WANG Y W, CAO B Z, et al. The suppression and promotion of DNA charge inversion by mixing counterions [J]. Soft matter, 2015, 11(20):40994105.
[9]van MEGEN W, UNDERWOOD S M. Dynamiclightscattering study of glasses of hard colloidal spheres [J]. Physical Review E （Statistical Physics Plasmas Fluids & Related Interdisciplinary Topics）, 1993, 47(1):248261.
[10]XIA W Y, WANG Y W, YANG A, et al. DNA Compaction and charge inversion induced by organic monovalent ions [J]. Polymers, 2017, 9(4):128.
[11]SUN L L, SUN Y J, XU F G, et al. Atomic force microscopy and surfaceenhanced Raman scattering detection of DNA based on DNAnanoparticle complexes [J]. Nanotechnology, 2009, 20(12):125502.
[12]冉诗勇，王艳伟，杨光参. 原子力显微镜扫描成像DNA分子[J].物理实验, 2011，31（11）:14.
[13]NECAS D, KLAPETEK P. Gwyddion: an opensource software for SPM data analysis [J]. Central European Journal of Physics, 2012, 10(1):181188.
[14]GUO Z L, WANG Y W, YANG A, et al. The effect of pH on charge inversion and condensation of DNA [J]. Soft Matter, 2016, 12(31): 66696674.
[15]PETTIT L D. Critical survey of formation constants of complexes of histidine, phenylalanine, tyrosine, LDOPA and tryptophan [J]. Pure & Applied Chemistry, 1984, 56(2):247292.
[16]LUAN B Q, AKSIMENTIEV A. Electric and electrophoretic inversion of the DNA charge in multivalent electrolytes [J]. Soft Matter, 2010, 6(2):243246.

[1]	葛俊伟, 李平, 张童童, 高飞, 王文越, 王钧楠, 孙稚颖. 山东新分布药用植物碎米桠的DNA条形码分子鉴定[J]. 山东科学, 2022, 35(4): 15-20.
[2]	刘淑丽,张金玉,李春晖,王晓丽. 基于达布变换的带三角势的Gross Pitaevskii方程的孤子解[J]. 山东科学, 2022, 35(3): 115-122.
[3]	徐敏,王艳伟,杨光参. 不同体积分数二甲基亚砜对DNA变性的影响[J]. 山东科学, 2022, 35(3): 131-137.
[4]	殷欣, 孟艺伟, 赵丽娅, 齐君, 夏雪奎, 高翠玲. 室内新风空气净化系统微生物多样性研究[J]. 山东科学, 2020, 33(5): 99-105.
[5]	李聪聪, 杨光参, 王艳伟. 不同价态的阴离子在DNA凝聚效应中的抑制作用[J]. 山东科学, 2020, 33(5): 119-126.
[6]	李树行, 祝泽栋, 徐紫颜, 杨光参. 不同pH环境下二价离子对聚电解质链的影响[J]. 山东科学, 2019, 32(4): 94-100.
[7]	陈洋，王艳伟，杨光参*. 基于原子力显微镜研究p53蛋白与PBR322 DNA的相互作用[J]. 山东科学, 2017, 30(4): 106-111.
[8]	郭子龙,王艳伟,杨光参. 铁离子引起的DNA电荷逆转和凝聚[J]. 山东科学, 2016, 29(4): 93-98.
[9]	张旭，王艳伟，杨光参. 壳聚糖与DNA相互作用的单分子方法研究[J]. 山东科学, 2014, 27(3): 1-6.
[10]	罗俊彩, 扈进冬, 李纪顺, 杨合同. 分子生物学方法在木霉菌分类研究中的应用[J]. J4, 2010, 23(4): 19-21.
[11]	范运梁, 崔春晓, 杨静, 姜庆, 刘雪, 戴美学. 两株花生根瘤菌的比较研究及结瘤能力的测定[J]. J4, 2010, 23(1): 22-27.

组氨酸对DNA电荷逆转和凝聚的影响

Histidine induced DNA charge inversion and condensation

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 11

Metrics

本文评价

推荐阅读 0