不同pH环境下二价离子对聚电解质链的影响

doi:10.3976/j.issn.1002-4026.2019.04.013

山东科学 ›› 2019, Vol. 32 ›› Issue (4): 94-100.doi: 10.3976/j.issn.1002-4026.2019.04.013

不同pH环境下二价离子对聚电解质链的影响

李树行，祝泽栋，徐紫颜，杨光参^*

温州大学数理与电子信息工程学院，浙江温州 325035

收稿日期:2019-04-15 出版日期:2019-08-20 发布日期:2019-08-07
通信作者: 杨光参，男，教授，研究方向为生命中的凝聚态物理。E-mail:yanggc@wzu.edu.cn E-mail:yanggc@wzu.edu.cn
作者简介:李树行（1989—），男，硕士研究生，研究方向为生命中的凝聚态物理。
基金资助:
国家自然科学基金（11574232,11274245）

Effect of divalent ions on the polyelectrolyte chains at different pH

LI Shu-hang, ZHU Ze-dong, XU Zi-yan, YANG Guang-can^*

School of Mathematics and Electronic Information Engineering, Wenzhou University, Wenzhou 325035, China

Received:2019-04-15 Online:2019-08-20 Published:2019-08-07

摘要/Abstract

摘要： 基于郎之万动力学方法，研究了不同pH环境下二价反离子对半柔性聚电解质链构象变化的影响。聚电解质用粗粒度的珠簧模型表示，在pH-pK>0时，回转半径几乎不变，随着pH-pK值的降低，聚电解质链的回转半径减小并且逐渐发生了缩合。在实验中，通过改变Mg2+介导下DNA溶液的pH环境，对DNA进行了原子力显微镜扫描成像，发现DNA在中性和弱酸性环境中保持自由松散状态，当pH降低到4以下，即酸性变强时DNA逐步缩合成球状并达到稳定，这一结果与模拟结果一致。

关键词: 郎之万动力学, 反离子, 聚电解质, pH, DNA

Abstract: In this study, the effect of divalent counterions on the conformational change of the semi-flexible polyelectrolyte chains was investigated at different pH based on Langevin’s dynamics. Polyelectrolyte is represented by a coarse-grained bead spring model, with the radius of gyration (Rg) remaining almost constant at pH-pK>0. Rg reduces and the polyelectrolyte chain shrinks as the value of pH-pK decreases. Meanwhile, in the experiment, the atomic force microscopy images of the DNA mediated by Mg²⁺ were obtained in different pH environments to denote that the DNA remained in a free and loose state in neutral and weakly acidic environments. At pH<4, i.e., strong acidity, DNA was gradually condensed into a spherical shape and stabilized. Further, the experimental result agrees with the simulation results.

Key words: Langevin’s dynamics, counter ion, polyelectrolyte, pH, DNA

中图分类号:

李树行, 祝泽栋, 徐紫颜, 杨光参. 不同pH环境下二价离子对聚电解质链的影响[J]. 山东科学, 2019, 32(4): 94-100.

LI Shu-hang, ZHU Ze-dong, XU Zi-yan, YANG Guang-can. Effect of divalent ions on the polyelectrolyte chains at different pH[J]. Shandong Science, 2019, 32(4): 94-100.

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不同pH环境下二价离子对聚电解质链的影响

Effect of divalent ions on the polyelectrolyte chains at different pH

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