J4 ›› 2014, Vol. 27 ›› Issue (3): 17-22.doi: 10.3976/j.issn.1002-4026.2014.03.004

• 中药与天然活性产物专栏 • 上一篇    下一篇

天麻素分子印迹聚合物的吸附和识别性能研究

陈凌霄1,2,马秀丽1,2,王晓2,耿岩玲2,高乾善2,谢宏凯1,2,纪文华2*   

  1. 1. 山东农业大学食品学院,山东 泰安 271000; 2. 山东省分析测试中心,山东 济南250014
  • 收稿日期:2014-03-20 出版日期:2014-06-20 发布日期:2014-06-20
  • 通信作者: 纪文华(1983-),男,硕士,研究方向为精细化学品的合成研究。 E-mail:jwh519@163.com
  • 作者简介:陈凌霄(1988-),男,硕士研究生,研究方向为天然产物分离纯化。Email: wsclx88@126.com

Absorption and recognition performance of gastrodin molecularly imprinted polymers

 CHEN Ling-Xiao1,2, MA Xiu-Li1,2, WANG Xiao2, GENG Yan-Ling2, GAO Qan-Shan2, XIE Hong-Kai1,2, JI Wen-Hua2*   

  1. 1. School of Food Science and Engineering, Shandong Agricultural University, Taian 271000, China; 2. Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China
  • Received:2014-03-20 Online:2014-06-20 Published:2014-06-20

摘要:

       采用分子印迹技术,以天麻素为模板,合成了对天麻素具有选择性吸附效果的分子印迹聚合物(MIPs)。扫描电镜和平衡吸附实验表明,与空白印迹聚合物(NIPs)相比,MIPs表面粗糙多孔并且有较多的结合位点和较高的吸附容量。在吸附动力学实验中,MIPs在30 min即可吸附最大吸附量的88.38%。在选择性实验中,MIPs对天麻素的吸附量远大于对其结构类似物葡萄糖的吸附量,体现了良好的选择性。研究结果表明,本材料有作为选择性填料来提取分离天麻素的潜能。

关键词: 分子印迹, 天麻, 天麻素, 吸附性能

Abstract:

       We synthesized molecularly imprinted polymers (MIPs) with selective absorption effect on gastrodin by molecular imprinting technology and gastrodin as a template. Scanning electron microscopy (SEM) and equilibrium absorption experiment reveal that MIPs are rough and porous and have many binding points higher absorption capacity, as compared with nonimprinted polymers (NIPs). MIPs can reach 88.38% of their equilibrium absorption capacity within 30 min in absorption kinetics. Absorption capacity of MIPs to gastrodin is much greater than to glucose, so they exhibit better selective absorption capacity. Results indicate that MIPs, selective stuffings, have such potency as extracting and separating gastrodin from natural sources.

Key words: molecularly imprinted polymer, gastrodia, gastrodin, absorption performance

中图分类号: 

  • R284|O657