基于DNS原理的还原糖测定仪

doi:10.3976/j.issn.1002-4026.2012.03.020

J4 ›› 2012, Vol. 25 ›› Issue (3): 95-97.doi: 10.3976/j.issn.1002-4026.2012.03.020

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A DNS based reducing sugar detector

GAO Gao-Heng¹, ZHOU Wan-Li², ZHU Si-Rong², BI Chun-Yuan², DIAO Xiao-Hua², SHI Jian-Guo²

1.Shandong Provincial Key Laboratory of Biosensors, Biotechnology Center, Shandong Academy of Sciences, Jinan 250014, China; 2.Institute of Biology, Shandong Academy of Sciences, Jinan 250014, China

Received:2012-01-06 Online:2012-06-20 Published:2012-06-20

Abstract

Abstract:

We designed a DNS based reducing sugar detector. It has such advantages as low-concentration reducing sugar detection, convenient operation, easy maintenance, high accuracy and good repeatability. It employs colorimetry to detect the differential light absorption value of dye liquor before and after heating and then to calculate the content of reducing sugar in a sample. We discover that the content of reducing sugar is proportional to the light absorption value. Glucose of 0.1% is used to calibrate measurement. The linear rate of more than 0.99 exists when the concentration range is between 0% and 0.1%. The detector can stably detect the content of reducing sugar in beer, sorbitol and ferment.

Key words: DNS, reducing sugar, colorimetry

CLC Number:

TP212.3

GAO Gao-Heng, ZHOU Wan-Li, ZHU Si-Rong, BI Chun-Yuan, DIAO Xiao-Hua, SHI Jian-Guo. A DNS based reducing sugar detector[J].J4, 2012, 25(3): 95-97.

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A DNS based reducing sugar detector

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