氨基酸分析仪法快速检测土鳖虫中谷氨酸含量

doi:10.3976/j.issn.1002-4026.2021.03.004

Shandong Science ›› 2021, Vol. 34 ›› Issue (3): 26-32.doi: 10.3976/j.issn.1002-4026.2021.03.004

• Traditional Chinese medicine and natural active products • Previous Articles Next Articles

Rapid determination of glutamic acid content in Eupolyphaga Steleophaga using an amino acid analyzer

ZHANG Hua-li¹, ZHOU Hong-liang², SUN Jia-chang¹

1.Collaborative Innovation Center for Birth Defect Research and Transformation of Shandong Province,Jining Medical University,Jining 272067,China; 2. Jining Maternal and Child Health Family Planning Service Center, Jining 272000, China

Received:2020-07-22 Online:2021-06-20 Published:2021-06-03

Abstract

Abstract:

The effects of various concentrations of hydrochloric acid, material-to-liquid ratios, and extraction times on the extraction of glutamate in Eupolyphaga Steleophaga were compared using an amino acid analyzer to establish a method to measure the glutamate content in Eupolyphaga Steleophaga. The results showed that the best extraction process for glutamic acid in Eupolyphaga Steleophaga was a material-to-liquid ratio of 1:1 (mg/mL), 6 mol/L hydrochloric acid, and hydrolysis time of 22 h. The recovery rate of the glutamic acid analyzer method was 99.2%, and the relative standard deviation of the precision test was 0.19%. There were significant differences in the glutamate content in Eupolyphaga Steleophaga from different regions, ranging from 1.068% to 1.149%. The method is reproducible, fast, efficient, and easy to perform. It is suitable for the detection of amino acid content in Eupolyphaga Steleophaga and can provide technical support for its quality control and in-depth resource development.

Key words: Eupolyphaga Steleophaga, glutamic acid, extraction method, amino acid analyzer

CLC Number:

R284. 2

ZHANG Hua-li, ZHOU Hong-liang, SUN Jia-chang.

Rapid determination of glutamic acid content in Eupolyphaga Steleophaga using an amino acid analyzer[J].Shandong Science, 2021, 34(3): 26-32.

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Rapid determination of glutamic acid content in Eupolyphaga Steleophaga using an amino acid analyzer

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