牛膝中齐墩果酸和β-蜕皮甾酮的HPLC法含量测定及指纹图谱研究

doi:10.3976/j.issn.1002-4026.2015.05.001

SHANDONG SCIENCE ›› 2015, Vol. 28 ›› Issue (5): 1-6.doi: 10.3976/j.issn.1002-4026.2015.05.001

• Traditional Chinese medicine and natural active products • Next Articles

Content determination of βecdysterone and oleanolic acid in Achyranthes bidentata Blume by HPLC and their fingerprints

ZHANG Minmin1,2,ZHAO Hengqiang1*,ZHOU Siduo1,3,WANG Daijie1WANG Xiao1,LIU Daicheng2,GENG Yanling1,MU Yuzhu4

1.Shandong Provincial Key Laboratory of Traditional Chinese Medicine Quality Control Technology, Shandong Analysis and Test Center,Shandong Academy of Sciences, Jinan 250014, China; 2. School of Life Sciences, Shandong Normal University, Jinan 250014, China;3. School of Food Science and Engineering, Shandong Agricultural University, Tai'an 271018, China;4.Yantai changui Pioneer Wine Co.Ltd.,Yantai 264000,China

Received:2015-04-14 Online:2015-10-20 Published:2015-10-20

Abstract

Abstract: We constructed quantitative high performance liquid chromatography (HPLC) fingerprint of Achyranthes bidentata Blume and determined the contents of oleanolic acid and βecdysterone. We analyzed 13 batches of Achyranthes bidentata Blume with Agilent ZORBAX SBC18 column, 0.6% formic acid as fluid phase, column temperature of 25 ℃ and detection wavelength of 260 nm. HPLC fingerprint of Achyranthes bidentata Blume included sixteen common peaks and four characteristic peaks. We also quantitatively determined two of these peaks, peak 5 and peak 7. From their similarity analysis, we primarily confirmed Achyranthes bidentata Blume from different origins had significant distinctions in quality. This research provides an approach and technology support for the quality control of Achyranthes bidentata.

Key words: HPLC, β-ecdysteronein, Achyranthes bidentata, oleanolic acid, fingerprint

CLC Number:

R284.1

ZHANG Minmin,ZHAO Hengqiang,ZHOU Siduo,WANG Daijie，WANG Xiao,LIU Daicheng,GENG Yanling,MU Yuzhu. Content determination of βecdysterone and oleanolic acid in Achyranthes bidentata Blume by HPLC and their fingerprints[J].SHANDONG SCIENCE, 2015, 28(5): 1-6.

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Content determination of βecdysterone and oleanolic acid in Achyranthes bidentata Blume by HPLC and their fingerprints

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