山东科学 ›› 2018, Vol. 31 ›› Issue (1): 55-.doi: 10.3976/j.issn.1002-4026.2018.01.010

• 生物传感器 • 上一篇    下一篇

生物传感器在玉米酶法浸泡工艺中的应用

刘庆艾,马恒,马耀宏,杨俊慧,孟庆军,杨艳,史建国*   

  1. 齐鲁工业大学(山东省科学院),山东省科学院生物研究所,山东省生物传感器重点实验室,山东 济南 250014
  • 收稿日期:2017-06-27 出版日期:2018-02-20 发布日期:2018-02-20
  • 通信作者: 史建国(1960—),男,研究员,研究方向为微生物学。E-mail: shijg@sdas.org E-mail:shijg@sdas.org
  • 作者简介:刘庆艾(1986—),女,博士,助理研究员,研究方向为微生物代谢。E-mail: liuqingai1986@126.com
  • 基金资助:

    山东省科学院青年科学基金(2015QN009);山东省自然科学基金中青年科学家科研奖励基金(ZR2016CB42);山东省科学院科技发展基金(科基合字2017第37号);国家高技术研究发展计划(863计划)(2015AA021005)

Application of biosensors in corn enzymatic steeping technology

LIU Qing-ai,MA Heng,MA Yao-hong,YANG Jun-hui,MENG Qing-jun,YANG Yan,SHI Jian-guo*   

  1. Shandong Provincial Key Laboratory of Biosensors , Biology Institute ,Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
  • Received:2017-06-27 Online:2018-02-20 Published:2018-02-20

摘要:

为了对玉米酶法浸泡过程中的生化参数进行控制,采用生物传感器对浸泡工艺过程的乳酸、葡萄糖、还原糖进行适时检测,并与滴定法、碘量法、费林试剂法进行比较。结果表明,生物传感器与传统方法检测结果相差不大,但是响应速度快、结果准确、测试成本低,且样品不需要复杂的预处理就可以直接测定。

关键词: 生化参数, 玉米酶法浸泡, 生物传感器

Abstract:

In order to control the biochemical parameters of the corn enzymatic steeping process, biosensor was used to timely detect the lactic acid, glucose and reducing sugar during corn soaking process, and  this method  was also compared with titration, iodimetry and fehling's  reagent. The results showed that there was little difference between the test results of biosensor and those of the traditional methods, but biosensor had many advantages, such as quick response, accurate result and low cost, and samples could be directly determined without complex pretreatment.

Key words: biochemical parameter, biosensor, corn enzymatic steeping

中图分类号: 

  • TS234