Abstract:

To optimize rapid propagation of tissue culture technique of Salvia miltiorrhiza Bge., we addressed the impact of its tissuecultured plantlets by different plant growth regulators and different culture conditions with tender stems with axillary bud as explants. Results show that the most effective induction to axillary buds is MS＋6BA(1.0 mg/L)＋NAA(0.5 mg/L)＋sucrose(3×104 mg/L)＋agar(7×103 mg/L). The most favorable rooting conditions of tissue culture seedling are 1/2MS＋IBA(1.0 mg/L)＋KT(1.0 mg/L)＋activated carbon(5 ×102 mg/L)＋sucrose(2×104 mg/L)＋agar(7×103 mg/L) and 22 ℃ . This technique can be applied to rapid propagation, rejuvenation and detoxification of Salvia miltiorrhiza Bge.

Key words: tissue culture, Salvia miltiorrhiza Bge., rapid propagation, conditions optimization