J4 ›› 2013, Vol. 26 ›› Issue (2): 61-65.doi: 10.3976/j.issn.1002-4026.2013.02.012

• Traditional Chinese medicine and natural active products • Previous Articles     Next Articles

Isolation of 6-gingerol, 8-gingerol and 6-shogaol from dry rhizome  of Zingiber officinale Rosc. by silica gel column chromatography and highspeed countercurrent chromatagraphy

 XU Mei-Xia, WANG Xiao, LIU Jian-Hua, GENG Yan-Ling, WANG Dai-Jie, LIU Feng   

  1. 1. College of Food Science and Engineering, Shandong Agricultural University, Taian 271000, China; 2. Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China
  • Received:2012-12-12 Published:2013-04-20 Online:2013-04-20

PDF (PC)

Like

Abstract:

We constructed the method of isolating 6gingerol, 8gingerol and 6shogaol from dry rhizome of Zingiber officinale Rosc. by silica gel column chromatography and highspeed countercurrent chromatagraphy based on experiments. We extracted the samples of Zingiber officinale Rosc. by ethyl acetate ultrasonic extraction and then coarsely treated them by silica gel column chromatography. We further selected fraction A3 and A4 to be separated by highspeed countercurrent chromatography(HSCCC). The purity of the obtained single ingredient was detected by highperformance liquid chromatography(HPLC) and the structure of the obtained compound was identified by electrospray ionization(ESIMS) and 1HNMR. Results show that the method has such positives as simplicity, rapidity, highpurity products, large amount of preparation and highefficiency separation.

Key words: Zingiber officinale Rosc., highspeed countercurrent chromatagraphy, silica gel column, 6-gingerol, 8-gingerol, 6-shogaol

CLC Number: 

  • R284.2

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0), which permits third parties to freely share (i.e., copy and redistribute the material in any medium or format) and adapt (i.e., remix, transform, or build upon the material) the articles published in this journal, provided that appropriate credit is given, a link to the license is provided, and any changes made are indicated. The material may not be used for commercial purposes. For details of the CC BY-NC 4.0 license, please visit: https://creativecommons.org/licenses/by-nc/4.0

鲁ICP备10205608号
Website Copyright © Editorial Office of Shandong Science
Tel: 0531-82605310　E-mail: xuxy@qlu.edu.cn
Powered by Beijing Magtech Co. Ltd