半夏泻心汤含药血清对胃癌细胞增殖影响的时效及量效关系研究

doi:10.3976/j.issn.1002-4026.20240035

Abstract

Abstract:

The pharmacological method of traditional Chinese medicine serum was used to explore the effect of Banxiaxiexin Decoction (BXD) on the proliferation of different gastric cancer cells in vitro. Additionally,the preparation conditions of BXD drug-containing serums were examined toobserve the pharmacodynamics of gastric cancer cells. Blood was collected at intervals of 30, 60, 90, 120, and 150 minutes after high, medium, and low intragastric gavage of BXD to prepare drug-containing serums. Based on the selected gavage doses and blood collection times, MGC803, MKN45, AGS, and HGC27 cells were exposed to 5%~50% concentrations of drug-containing serum, and CCK8 assay was employed to detect the inhibitory effects of different gavage doses, blood collection times, and volume fractions of BXD drug-containing serum on the proliferation of gastric cancer cells. The test results showed that compared with other time points, all BXD drug-containing serums had the strongest inhibitory effect on gastric cancer cells at the blood collection time of 120 minutes. Compared with the low-dose BXD group, the medium-dose BXD group had a strong inhibitory effect on gastric cancer cells, and there was no significant difference between the medium-dose and high-dose BXD groups. Based on the analysis of the inhibitory effects of 10 concentrations ranging from 5% to 50% on different gastric cancer cells, the IC₅₀ values of all drug-containing serums collected 120 minutes after gavage in the medium-dose BXD group were 20%. Analysis of the inhibitory effects of different gavage doses, blood collection times, and volume fractions on different gastric cancer cells revealed that a medium dose of BXD, blood collection time of 120 minutes,and a volume fraction of 20% had the strongest inhibitory effect on gastric cancer cells. Therefore, the medicated serum with a blood collection time of 120 minutes and a volume fraction of 20% in the medium-dose BXD group had the best pharmacodynamic effect on gastric cancer cells.

Key words: Banxiaxiexin Decoction-containing serum, gastric cancer, cell proliferation, Chinese medicine serum pharmacology, time-effect, dose-effect

CLC Number:

R285

ZU Guoxiu, LIU Xijian, ZU Xiuli, TANG Jiqin, HUANG Hailiang, HAN Tao. Time-effect and dose-effect relationship study on the effect of Banxiaxiexin Decoction-containing serum on the proliferation of gastric cancer cells[J].Shandong Science, 2025, 38(1): 9-22.

Figures/Tables 20

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Table 1

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References 15

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组别	体积分数/%	24 h		48 h		72 h
组别	体积分数/%	平均值±标准差	增殖率/%	平均值±标准差	增殖率/%	平均值±标准差	增殖率/%
对照组	0	2.094±0.128	100	2.729±0.036	100	3.163±0.029	100
BXD组	5	2.080±0.014	99.27	2.656±0.039	97.23	2.792±0.051^**	87.98
	10	1.847±0.012^*	87.70	2.361±0.063^**	86.09	2.508±0.028^**	78.77
	15	1.592±0.014^**	75.02	2.040±0.039^**	73.91	2.195±0.030^**	68.61
	20	1.149±0.014^**	52.98	1.530±0.023^**	54.62	1.779±0.039^**	55.09
	25	0.963±0.047^**	43.74	1.295±0.058^**	45.74	1.344±0.039^**	40.98
	30	0.882±0.031^**	39.70	1.007±0.061^**	34.83	1.044±0.066^**	31.24
	35	0.661±0.021^**	28.70	0.843±0.050^**	28.62	1.121±0.051^**	33.74
	40	0.477±0.008^**	19.54	0.538±0.019^**	17.09	0.607±0.065^**	17.06
	45	0.355±0.011^**	13.51	0.368±0.018^**	10.65	0.387±0.027^**	9.92
	50	0.293±0.010^**	10.42	0.211±0.014^**	4.71	0.191±0.020^**	3.55

组别	体积分数/%	24 h		48 h		72 h
组别	体积分数/%	平均值±标准差	增殖率/%	平均值±标准差	增殖率/%	平均值±标准差	增殖率/%
对照组	0	2.050±0.044	100	2.469±0.004	100	3.333±0.008	100
BXD组	5	1.933±0.007^*	94.01	2.455±0.016	99.43	2.994±0.028^**	89.58
	10	1.628±0.024^**	78.48	1.983±0.023^**	79.57	2.791±0.014^**	83.30
	15	1.477±0.003^**	70.75	1.609±0.027^**	63.86	2.504±0.019^**	74.47
	20	1.316±0.010^**	62.54	1.382±0.064^**	54.30	1.600±0.013^**	46.63
	25	1.196±0.021^**	56.43	1.045±0.016^**	40.14	1.419±0.007^**	41.09
	30	1.065±0.032^**	49.73	0.814±0.013^**	30.42	1.066±0.023^**	30.21
	35	0.897±0.011^**	41.17	0.645±0.016^**	23.32	0.754±0.027^**	20.59
	40	0.661±0.008^**	29.13	0.529±0.027^**	18.46	0.654±0.016^**	17.51
	45	0.514±0.007^**	21.63	0.417±0.024^**	13.72	0.425±0.030^**	10.47
	50	0.343±0.006^**	12.92	0.175±0.017^**	3.54	0.159±0.022^**	2.26

组别	体积分数/%	24 h		48 h		72 h
组别	体积分数/%	平均值±标准差	增殖率/%	平均值±标准差	增殖率/%	平均值±标准差	增殖率/%
对照组	0	1.801±0.019	100	2.507±0.018	100	3.102±0.011	100
BXD组	5	1.674±0.010^**	92.61	2.469±0.017	98.42	2.894±0.018^**	93.16
	10	1.564±0.054^**	86.24	2.090±0.022^**	82.66	2.669±0.007^**	85.70
	15	1.413±0.011^**	77.44	1.951±0.010^**	76.91	2.415±0.002^**	77.31
	20	1.073±0.011^**	57.65	1.409±0.017^**	54.41	1.676±0.016^**	52.92
	25	0.795±0.036^**	41.52	1.109±0.008^**	41.92	1.233±0.007^**	38.27
	30	0.626±0.025^**	31.65	1.079±0.003^**	40.68	1.023±0.011^**	31.34
	35	0.537±0.023^**	26.47	0.909±0.045^**	33.64	0.750±0.016^**	22.33
	40	0.427±0.019^**	20.08	0.719±0.019^**	25.72	0.480±0.017^**	13.41
	45	0.316±0.016^**	13.62	0.424±0.022^**	13.51	0.359±0.020^**	9.41
	50	0.188±0.024^**	6.17	0.223±0.033^**	5.13	0.149±0.003^**	2.48

组别	体积分数/%	24 h		48 h		72 h
组别	体积分数/%	平均值±标准差	增殖率/%	平均值±标准差	增殖率/%	平均值±标准差	增殖率/%
对照组	0	1.249±0.017	100	1.551±0.004	100	2.802±0.011	100
BXD组	5	1.174±0.020^**	93.47	1.550±0.014	99.95	2.578±0.007^**	91.77
	10	1.129±0.029^**	89.43	1.351±0.016^**	86.37	2.472±0.015^**	87.85
	15	0.828±0.011^**	62.99	1.257±0.004^**	79.97	2.092±0.002^**	73.89
	20	0.679±0.012^**	49.82	0.898±0.005^**	55.56	1.583±0.012^**	55.14
	25	0.548±0.009^**	38.31	0.727±0.001^**	43.91	1.334±0.010^**	45.99
	30	0.523±0.017^**	36.14	0.623±0.016^**	36.84	1.307±0.003^**	45.01
	35	0.373±0.013^**	22.93	0.555±0.024^**	32.22	1.065±0.003^**	36.08
	40	0.301±0.012^**	16.61	0.403±0.017^**	21.83	0.546±0.025^**	17.00
	45	0.287±0.008^**	15.37	0.316±0.016^**	15.92	0.440±0.011^**	13.10
	50	0.197±0.002^**	7.40	0.229±0.008^**	10.02	0.337±0.003^**	9.29

组别	体积分数/%	OD值(平均值±标准差)
组别	体积分数/%	30 min		60 min		90 min		120 min		150 min
对照组	0	1.366±0.022
BXD低剂量组	10		1.237±0.053^*		1.358±0.012		1.304±0.008^*		1.321±0.056		1.45±0.047^*
	20		1.252±0.093		1.282±0.050		1.304±0.018^*		1.221±0.029^**		1.23±0.018^**
BXD中剂量组	10		1.209±0.058^*		1.182±0.054^**		1.11±0.111^**		1.163±0.05^**		1.217±0.035^**
	20		1.154±0.069^**		1.191±0.025^**		1.175±0.062^**		1.003±0.028^**		1.142±0.053^**
BXD高剂量组	10		1.273±0.007^**		1.201±0.014^**		1.351±0.031		1.323±0.017		1.316±0.033
	20		1.233±0.037^**		1.264±0.055^*		1.214±0.020^**		1.172±0.048^**		1.243±0.074

Time-effect and dose-effect relationship study on the effect of Banxiaxiexin Decoction-containing serum on the proliferation of gastric cancer cells

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组别	体积分数/%	OD值(平均值±标准差)
组别	体积分数/%	30 min	60 min	90 min	120 min	150 min
对照组	0	1.23±0.038
BXD低剂量组	10	1.254±0.010	1.225±0.024	1.24±0.017	1.267±0.010	1.181±0.033
	20	1.212±0.026	1.222±0.012	1.206±0.03	1.164±0.011^*	1.195±0.002
BXD中剂量组	10	1.166±0.034	1.112±0.028^*	1.089±0.041^*	1.124±0.011^**	1.138±0.007^*
	20	1.106±0.036^*	1.113±0.014^**	1.056±0.005^**	1.05±0.005^**	1.125±0.018^*
BXD高剂量组	10	1.21±0.011	1.135±0.022^*	1.214±0.077	1.151±0.004^*	1.162±0.013^*
	20	1.127±0.047^*	1.131±0.006^*	1.138±0.011^*	1.072±0.008^**	1.139±0.006^*

组别	体积分数/%	OD值(平均值±标准差)
组别	体积分数/%	30 min	60 min	90 min	120 min	150 min
对照组	0	1.739±0.064
BXD低剂量组	10	1.767±0.120	1.675±0.023	1.579±0.058^*	1.423±0.130^*	1.446±0.051^**
	20	1.634±0.044	1.461±0.034^**	1.425±0.035^**	1.241±0.018^**	1.368±0.044^**
BXD中剂量组	10	1.844±0.009^*	1.773±0.042	1.408±0.011^**	1.241±0.024^**	1.288±0.013^**
	20	1.614±0.048	1.305±0.062^**	1.154±0.019^**	1.088±0.007^**	1.175±0.038^**
BXD高剂量组	10	1.826±0.029	1.736±0.075	1.408±0.045^**	1.238±0.006^**	1.288±0.119^**
	20	1.743±0.037	1.443±0.043^**	1.296±0.015^**	1.19±0.004^**	1.29±0.005^**

组别	体积分数/%	OD值(平均值±标准差)
组别	体积分数/%	30 min	60 min	90 min	120 min	150 min
对照组	0	1.542±0.012
BXD低剂量组	10	1.552±0.050	1.459±0.005^**	1.474±0.014^*	1.444±0.004^**	1.527±0.006
	20	1.567±0.015	1.464±0.030	1.431±0.008^**	1.379±0.039^**	1.474±0.008^**
BXD中剂量组	10	1.477±0.004^**	1.42±0.021^**	1.393±0.006^**	1.375±0.007^**	1.405±0.004^**
	20	1.403±0.020^**	1.381±0.016^**	1.348±0.005^**	1.297±0.021^**	1.347±0.010^**
BXD高剂量组	10	1.5±0.019^*	1.538±0.029	1.593±0.028^*	1.558±0.024	1.634±0.017^**
	20	1.473±0.019^**	1.527±0.021	1.428±0.023^**	1.619±0.029^*	1.611±0.043