Abstract:

To investigate the diversity of microbial communities and to obtain culturable strains from healthy and rusty root-rot rhizosphere soil samples of Panax quinquefolius L., it is necessary to provide a reference for the occurrence mechanism and prevention and control strategies for rusty root-rot. Traditional isolation and high-throughput sequencing were used for analyzing the healthy and rusty root-rot soil samples of Panax quinquefolius L. to explore their structural composition and diversity. In total, 24 strains of bacteria and 25 strains of fungi were isolated using the traditional isolation techniques, and the dominant genera were Pseudomonas, Bacillus, and Penicillium. The results of Illumina Miseq high-throughput sequencing revealed that Cyanobacteria and Proteobacteria dominated the bacterial communities in the healthy and rusty root-rot soil samples. Notably, the phylum proportions of Cyanobacteria, Proteobacteria, and Tenericutes in the rusty root-rot soil samples significantly decreased, whereas those of Bacteroidetes, Actinobacteria, Firmicutes, and Acidobacteria significantly increased. Furthermore, the bacterial diversity of the infected rusty root-rot rhizosphere soil decreased significantly. In conclusion, the composition and quantity of soil microbial communities were significantly different between the healthy and rusty root-rot soil samples, and this provides a theoretical basis for the study of the occurrence mechanism and prevention and control strategies for rusty root-rot Panax quinquefolium L.

Key words: Panax quinquefolium L., rhizosphere soil, bacteria and fungi, rusty root-rot, high-throughput sequencing, traditional isolation methods