不同体积分数二甲基亚砜对DNA变性的影响

doi:10.3976/j.issn.1002-4026.2022.03.016

摘要/Abstract

摘要：

利用原子力显微镜和动态光散射研究不同体积分数二甲基亚砜(DMSO)对质粒及线性DNA变性的影响。结果表明,体积分数1%的DMSO也能导致DNA发生局部变性,这个结果可以用原子力显微镜直接观测。同时,由于质粒DNA连接数的守恒以及DNA单链的产生,通过原子力显微镜和动态光散射发现,DNA随DMSO体积分数的增加逐渐形成超螺旋结构,DNA聚拢程度增加;DMSO体积分数从0增加到10%时,pBR322 DNA、5 000 bp DNA及λ-DNA的平均粒径分别从(474±10) nm、(554±11) nm和(871±17) nm降低到(257±8) nm、(282±18) nm和(449±21) nm。

关键词: DNA, 变性, DNA拓扑结构, 有机变性试剂, 粒径

Abstract:

The effects of different volume fractions of dimethyl sulfoxide (DMSO) on plasmid and linear DNA denaturation were investigated using atomic force microscopy and dynamic light scattering. It was found that a low volume fraction of 1% DMSO solution can also induce local DNA denaturation, which can be directly observed by atomic force microscopy. Simultaneously, due to the limitations of plasmid DNA linking number and single-stranded DNA generation, atomic force microscopy and dynamic light scattering showed that DNA gradually forms a superhelix structure with an increase in the volume fraction of DMSO. The average particle size of pBR322 DNA, 5 000 base pair DNA, and λ-DNA decreased from about (474±10) nm, (554±11) nm, and (871±17) nm, respectively, in 0 DMSO solution to (257±8) nm, (282±18) nm, and (449±21) nm, respectively, in 10% DMSO solution.

Key words: :DNA, denaturation, DNA topology structure, organic denaturation reagent, particle size

中图分类号:

徐敏,王艳伟,杨光参. 不同体积分数二甲基亚砜对DNA变性的影响[J]. 山东科学, 2022, 35(3): 131-137.

XU Min,WANG Yan-wei,YANG Guang-can. Effects of different volume fractions of dimethyl sulfoxide on DNA denaturation[J]. Shandong Science, 2022, 35(3): 131-137.

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