山东科学 ›› 2020, Vol. 33 ›› Issue (3): 53-61.doi: 10.3976/j.issn.1002-4026.2020.03.008

• 中药与天然活性产物 • 上一篇    下一篇

猪皮胶原肽-锌螯合物的制备工艺研究

吴家劲,边智杰,朱悦,张学涛,庞荔丹,高鹏   

  1. 山东中医药大学 药学院,山东 济南 250355
  • 出版日期:2020-05-29 发布日期:2020-06-01
  • 通信作者: 高鹏(1978—),男,副教授,硕士生导师,研究方向为中药新制剂、新剂型研究。E-mail:48395504@qq.com
  • 作者简介:吴家劲(1998—),男,研究方向为中药学。E-mail:410361804@qq.com
  • 基金资助:
    国家级大学生创新训练计划(201910441013)

Investigation on preparation process of pigskin collagen peptide-zinc chelate

WU Jia-jin, BIAN Zhi-jie, ZHU Yue,ZHANG Xue-tao,PANG Li-dan, GAO Peng   

  1. School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
  • Online:2020-05-29 Published:2020-06-01

摘要: 研究猪皮胶原肽-锌螯合物的制备工艺。以猪皮为原料,制备猪皮胶原肽-锌螯合物;以螯合率为指标,研究了猪皮胶原肽与锌质量比、pH、温度、时间对螯合率的影响;通过单因素试验、响应面法试验考察优化猪皮胶原肽的富锌工艺。结果显示,猪皮肽-锌螯合的最佳工艺为猪皮肽与锌质量比2:1,螯合pH 7.0,螯合温度50 ℃,螯合时间50 min,在此条件下,试验螯合率最佳,为69.27%,与模型预测值69.92%相近。该研究为新型生物肽锌螯合物的研制、猪皮的高值化利用提供了依据。

关键词: 猪皮胶原肽, 酶解, 锌元素, 螯合工艺, 响应面法

Abstract: This paper studies the preparation process of pigskin collagen peptide-zinc chelate. Pigskin was used as a raw material to prepare the pigskin collagen peptide-zinc chelate, and the effect of mass ratio of pigskin collagen peptide and zinc, pH, temperature, and time on the chelating rate, which was used as the index, was studied. We used a single-factor method and the response surface method to investigate zinc-rich technology of pigskin peptide. The results showed that the best technology for pigskin peptide-zinc chelation involved pigskin peptide-zinc mass ratio of 2:1 and a process with pH 7.0, 50 ℃, and 50 min for the chelation, resulting in the highest chelating rate of 69.27%, which was close to the predicted 69.92%. This study provides a basis for the development of new biological peptide-zinc chelation and the maximum utilization of pigskin.

Key words: pigskin collagen peptide, enzymatic hydrolysis, zinc, chelation process, response surface methodology

中图分类号: 

  • R284.2

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