草莓炭疽病拮抗木霉菌筛选、鉴定及功能评价

doi:10.3976/j.issn.1002-4026.2025027

摘要/Abstract

摘要： 针对当前草莓炭疽病发生严重并且难防难治的问题，本文以引起草莓炭疽病的暹罗炭疽为靶标，采用平板对峙、平板对扣等方法，筛选获得高效防治草莓炭疽病的生防木霉菌株HB 23422，经分子生物学和形态学特征观察鉴定为类棘孢木霉。该菌在PDA平板上对暹罗炭疽菌CM9具有强烈抑制作用，抑制率可达74.55%；离体叶片试验和盆栽试验表明该菌的孢子悬液可有效降低草莓炭疽病发病率。类棘孢木霉HB 23422生长速度快，产孢能力强，防治效果好，具有开发成微生物农药用于草莓炭疽病绿色防治的潜力。

关键词: 暹罗炭疽菌, 木霉, 分离鉴定, 抑菌率

Abstract: In response to the severe incidence and management challenges of strawberry anthracnose, this study targeted Colletotrichum siamense, the pathogen responsible for the disease. We used dual culture and sealed-plate assays to screen for the effective biocontrol Trichoderma strain HB 23422, which exhibited strong antagonistic activity against strawberry anthracnose, and was identified based on morphological characteristics and molecular biology as Trichoderma asperellum. On PDA plates, HB 23422 exhibited a strong inhibitory effect on Colletotrichum Siamense CM9, with an inhibition rate of more than 74.55%. Vitro itro leaf experiments and pot experiments showed that the spore suspension of this strain could effectively reduce the incidence of strawberry anthracnose. The strain HB 23422 grows rapidly, produces abundant spores, and has excellent disease control efficacy, indicating its potential for development as microbial pesticide for environment friendly control of strawberry anthracnose.

Key words: Colletotrichum siamense, Trichoderma, Separation and identification, Inhibition rate

中图分类号:

S182

刘建华, 蒋艳青, 李红梅, 杨建闻, 魏艳丽, 刘宝军, 李纪顺, 扈进冬. 草莓炭疽病拮抗木霉菌筛选、鉴定及功能评价[J]. 山东科学, 0, (): 1-.

LIU Jianhua, JIANG Yanqing, LI Hongmei, Yang Jianwen, WEI Yanli, LIU Baojun, LI Jishun, HU Jindong. Screening, identification and functional evaluation of trichoderma strains antagonistic to strawberry anthracnose [J]. Shandong Science, 0, (): 1-.

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