菝葜多糖分离纯化工艺研究

doi:10.3976/j.issn.1002-4026.2021.06.001

摘要/Abstract

摘要：

为了优选菝葜多糖的分离纯化工艺,以多糖纯度、多糖出膏率与吸附率等为指标,考察醇沉静置温度、醇沉静置时间与大孔吸附树脂型号等因素,确定菝葜多糖的最佳醇沉工艺与大孔吸附树脂纯化工艺。得到菝葜多糖最佳醇沉工艺为取含生药1.0 g/mL的药液,加入乙醇,使乙醇体积分数达到80%,醇沉1次,室温25 ℃静置12 h,抽滤得醇沉物,70 ℃干燥;纯化工艺为采用AB-8型大孔吸附树脂,用1 BV的2.0 mg/mL(以粗多糖计)的上样液,以2 BV/h的流速上样,再用3 BV的纯水以3 BV/h的流速进行洗脱。结果表明该优选工艺稳定可靠,可用于菝葜多糖的分离纯化。

关键词: 菝葜, 多糖, 醇沉, 大孔吸附树脂

Abstract:

The purpose of this study is to optimize the polysaccharide purification technique from Smilacis Chinae Rhizoma. With the purity, paste rate, adsorption rate, and other parameters as indicators, the optimal alcohol precipitation process and macroporous resin purification technique were determined by investigating the alcohol precipitation temperature, alcohol precipitation time, macroporous resin type, and other factors. The optimum alcohol precipitation process is as follows. Take 1.0 g/mL solution and add ethanol to make the alcohol content reach 80%. Then, perform alcohol precipitation once and leave it at 25 ℃ for 12 h. Finally, pass it through a suction filter to obtain alcohol precipitate and dry it at 70 ℃. Optimum purification technique is as follows. Load 1 BV sample (2.0 mg/mL) and pass it through AB-8 resin at the rate of 2 BV/h, followed by elution through 3 BV pure water at the rate of 3 BV/h. The results show that the optimized process is stable and reliable and can be used for purifying.

Key words: Smilacis Chinae Rhizoma, polysaccharide, alcohol precipitation, macroporous resin

中图分类号:

R284.2

梅明,周进,张毅,马良鹏,杨明,胡松. 菝葜多糖分离纯化工艺研究[J]. 山东科学, 2021, 34(6): 1-8.

Ming MEI,Jin ZHOU,Yi ZHANG,Liang-peng MA,Ming YANG,Song HU. Isolation and purification of polysaccharide from Smilacis Chinae Rhizoma[J]. Shandong Science, 2021, 34(6): 1-8.

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参考文献 19

[1]	全国中草药汇编编写组. 全国中草药汇编(上册)[M]. 北京: 人民卫生出版社, 1976: 752.
	National compilation group of Chinese herbal medicine. National compilation of Chinese herbal medicine (Volume 1)[M]. Beijing: People's Health Publishing House, 1976: 752.
[2]	吕翼, 吕金清, 裴学军. 药用金刚藤名实考证[J]. 河北中医杂志, 2002, 24(5):47-48.
	LÜ Y, LÜ J Q, PEI X J. Textual research on the name of Smilacis chinae rhizoma[J]. Hebei Journal of Traditional Chinese Medicine, 2002, 24(5):47-48.
[3]	国家药典委员会. 中华人民共和国药典2015年版一部[S]. 北京: 中国医药科技出版社, 2015: 308.
	Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China 2015 Volume 1[M]. Beijing: China Medical Science and Technology Press, 2015: 308.
[4]	齐进, 崔颖娜. 金刚藤多糖对慢性盆腔炎大鼠炎症介质、细胞凋亡及免疫细胞功能的影响[J]. 海南医学院学报, 2018, 24(13):1219-1221. DOI: 10.13210/j.cnki.jhmu.20180515.002. doi: 10.13210/j.cnki.jhmu.20180515.002
	QI J, CUI Y N. Effects of smilax bockii warb polysaccharide on inflammatory mediators,apoptosis and immune cell function in rats with chronic pelvic inflammatory disease[J]. Journal of Hainan Medical University, 2018, 24(13):1219-1221. DOI: 10.13210/j.cnki.jhmu.20180515.002. doi: 10.13210/j.cnki.jhmu.20180515.002
[5]	吴大章, 苏泰安, 代华均, 等. 金刚藤多糖对大鼠慢性盆腔炎的治疗作用及对血清免疫球蛋白的影响[J]. 中药药理与临床, 2015, 31(4):127-129. DOI: 10.13412/j.cnki.zyyl.2015.04.039. doi: 10.13412/j.cnki.zyyl.2015.04.039
	WU D Z, SU T A, DAI H J, et al. Therapeutic effect of Smilacis chinae rhizoma polysaccharide on chronic pelvic inflammatory disease in rats and its effect on serum immunoglobulin[J]. Pharmacology and Clinics of Chinese Materia Medica, 2015, 31(4):127-129. DOI: 10.13412/j.cnki.zyyl.2015.04.039. doi: 10.13412/j.cnki.zyyl.2015.04.039
[6]	陈东生, 吕永宁, 王杰. 菝葜的抗炎作用[J]. 中国医院药学杂志, 2000, 20(9):544-545.
	CHEN D S, LÜ Y N, WANG J. Study on the anti-inflaminatory effect of Smilax china L.[J]. Chinese Journal of Hospital Pharmacy, 2000, 20(9):544-545.
[7]	王立红, 何轩辉, 马涛, 等. 中药复方GCRF水提醇沉工艺的优化及对瘀热心梗大鼠心功能的改善[J]. 中国新药杂志, 2019, 28(7):862-870.
	WANG L H, HE X H, MA T, et al. Optimization of water extraction and alcohol precipitation process and improvement of cardiac function in rats with myocardial infarction (stasis-heat syndrome) for GCRF formula[J]. Chinese Journal of New Drugs, 2019, 28(7):862-870.
[8]	丛婧, 王秀丽. 响应曲面法优选人参葛根煎膏醇沉工艺[J]. 中国药业, 2019, 28(16):17-19.
	CONG J, WANG X L. Optimization of ethanol precipitation process of Panax Ginseng and Pueraria Lobata concentrated decoction by response surface method[J]. China Pharmaceuticals, 2019, 28(16):17-19.
[9]	董建新, 俞涵, 姜伟, 等. 响应面法优化白及多糖的提取和醇沉工艺[J]. 基因组学与应用生物学, 2019, 38(8):3681-3689. DOI: 10.13417/j.gab.038.003681. doi: 10.13417/j.gab.038.003681
	DONG J X, YU H, JIANG W, et al. Optimization of extraction and alcohol precipitation process of Bletilla striata polysaccharides based on response surface methodology[J]. Genomics and Applied Biology, 2019, 38(8):3681-3689. DOI: 10.13417/j.gab.038.003681. doi: 10.13417/j.gab.038.003681
[10]	罗开沛, 李小芳, 林浩, 等. 山香圆叶提取液纯化工艺的优化[J]. 中成药, 2017, 39(4):727-731.
	LUO K P, LI X F, LIN H, et al. Optimization of purification of extract from Turpinia arguta leaves[J]. Chinese Traditional Patent Medicine, 2017, 39(4):727-731.
[11]	冯素香, 苗明三, 苗晋鑫, 等. AB-8大孔吸附树脂同时分离纯化毛冬青总黄酮、总皂苷工艺[J]. 中国实验方剂学杂志, 2012, 18(3):5-8. DOI: 10.13422/j.cnki.syfjx.2012.03.037. doi: 10.13422/j.cnki.syfjx.2012.03.037
	FENG S X, MIAO M S, MIAO J X, et al. Simultaneous separation and purification technology of total flavonoids and total saponins from Llex pubescens by AB-8 macroporous resin[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2012, 18(3):5-8. DOI: 10.13422/j.cnki.syfjx.2012.03.037. doi: 10.13422/j.cnki.syfjx.2012.03.037
[12]	杨丽, 王宏伟, 李洪波, 等. 大孔树脂法富集黄芩籽种壳总黄酮[J]. 医药导报, 2017, 36(4):420-424.
	YANG L, WANG H W, LI H B, et al. Enrichment of total flavonoids from seed shell of Scutellaria Baicalensis by macroporous resin[J]. Herald of Medicine, 2017, 36(4):420-424.
[13]	梅明, 杨全伟, 马良鹏, 等. 红梅消总皂苷分离纯化及体外抗凝血活性研究[J]. 现代中药研究与实践, 2018, 32(4):36-39. DOI: 10.13728/j.1673-6427.2018.04.010. doi: 10.13728/j.1673-6427.2018.04.010
	MEI M, YANG Q W, MA L P, et al. Isolation and purification of Rubus parvifolius L.and its anti-coagulant activity in vitro[J]. Research and Practice on Chinese Medicines, 2018, 32(4):36-39. DOI: 10.13728/j.1673-6427.2018.04.010. doi: 10.13728/j.1673-6427.2018.04.010
[14]	王维. 桑叶多糖提取与纯化工艺的研究[D]. 西安:陕西科技大学, 2006. DOI: 10.7666/d.y973741. doi: 10.7666/d.y973741
	WANG W. Study on the extraction and purifiying technology of polysaccharide in morus alba leaves[D]. Xi'an: Shaanxi University of Science & Technology, 2006. DOI: 10.7666/d.y973741. doi: 10.7666/d.y973741
[15]	刘富岗, 李汉伟, 郑群杰, 等. 响应面法优选泽兰多糖的大孔吸附树脂纯化工艺[J]. 暨南大学学报(自然科学与医学版), 2019, 40(1):1-9. DOI: 10.11778/j.jdxb.2019.01.001. doi: 10.11778/j.jdxb.2019.01.001
	LIU F G, LI H W, Z Q J, et al. Optimization of purification process of Lycopus lucidus polysaccharide with macroporous adsorption resins by response surface method[J]. Journal of Jinan University(Natural Science & Medicine Edition), 2019, 40(1):1-9. DOI: 10.11778/j.jdxb.2019.01.001. doi: 10.11778/j.jdxb.2019.01.001
[16]	赵芷萌, 赵宏, 王宇亮, 等. 百合多糖的纯化及其对肠道菌群失调小鼠的调节作用[J]. 食品工业科技, 2020, 41(8):295-300. DOI: 10.13386/j.issn1002-0306.2020.08.047. doi: 10.13386/j.issn1002-0306.2020.08.047
	ZHAO Z M, ZHAO H, WANG Y L, et al. Purification of lily polysaccharide and its regulatory effect on intestinal flora dysregulation mice[J]. Science and Technology of Food Industry, 2020, 41(8):295-300. DOI: 10.13386/j.issn1002-0306.2020.08.047. doi: 10.13386/j.issn1002-0306.2020.08.047
[17]	刘富岗, 冯素香, 张杰, 等. 白屈菜多糖纯化工艺的优化[J]. 中成药, 2018, 40(2):475-477. DOI: 10.3969/j.issn.1001-1528.2018.02.048. doi: 10.3969/j.issn.1001-1528.2018.02.048
	LIU F G, FENG S X, ZHANG J, et al. Optimization of purification process of Polysaccharides from Chelidonium majus[J]. Chinese Traditional Patent Medicine, 2018, 40(2):475-477. DOI: 10.3969/j.issn.1001-1528.2018.02.048. doi: 10.3969/j.issn.1001-1528.2018.02.048
[18]	汤如莹, 王玉杰, 李伟, 等. D101型大孔吸附树脂分离纯化沙苑子总黄酮工艺研究[J]. 中草药, 2017, 48(16):3342-3346. DOI: 10.7501/j.issn.0253-2670.2017.16.014. doi: 10.7501/j.issn.0253-2670.2017.16.014
	TANG R Y, WANG Y J, LI W, et al. Purification of total flavonoids extracted from Astragali Complanati Semen using D-101 macroporous adsorption resin[J]. Chinese Traditional and Herbal Drugs, 2017, 48(16):3342-3346. DOI: 10.7501/j.issn.0253-2670.2017.16.014. doi: 10.7501/j.issn.0253-2670.2017.16.014
[19]	国家药品监督管理局. 药品注册管理办法[EB/OL]. (2020-03-30 )[2020-08-03]. https://www.nmpa.gov.cn/directory/web/nmpa/xxgk/fgwj/bmgzh/20200330180501220.html.
	National Medical Products Administration. Measures for the administration of drug registration[EB/OL]. (2020-03-30 )[2020-08-03]. https://www.nmpa.gov.cn/directory/web/nmpa/xxgk/fgwj/bmgzh/20200330180501220.html.

时间/h	多糖纯度/%	多糖出膏率/%
6 12 24	46.21 51.54 52.12	1.42 2.12 2.14
48	52.14	2.15

提取液生药质量浓度/(g·mL^-1)	多糖纯度/%	多糖出膏率/%
0.5 0.8 1.0	43.95 46.81 51.89	1.97 2.09 2.15
1.2	51.81	2.07

乙醇体积分数/%	多糖纯度/%	多糖出膏率/%
60 70 80	32.92 49.12 51.23	1.87 1.98 2.15
90	51.25	2.13

项目		多糖纯度/%	多糖出膏率/%
编号	1 2 3	51.51 52.47 51.32	2.13 2.11 2.12
均值		51.77	2.12

上样量/BV	吸附率/%
1	69.2
2	65.5
3	59.2
4	55.3
5	49.8