槲皮素对阿霉素所致肾病综合征大鼠的保护作用及机制

doi:10.3976/j.issn.1002-4026.2023.04.008

Abstract

Abstract:

To investigate the protective effect of quercetin on nephrotic syndrome model rats. Specific Pathogen Free (SPF) male rats were selected, and a single tail vein injection of adriamycin 6.5 mg/kg was used to induce nephrotic syndrome in the rat model. Urine was collected to determine 24 h urine protein concentrations, and the contents of blood biochemical serum total protein and albumin, total cholesterol, triglyceride levels, and renal function markers (blood urea nitrogen and serum creatinine) were analyzed. The pathological changes in renal tissue were observed by hematoxylin and eosin staining. Transmission electron microscopy was used to examine the ultrastructure of renal podocytes. Western blot was used to detect the expression of desmin, nephrin, and synaptopodin in renal tissue. The results showed that quercetin effectively alleviated 24 h urinary protein in nephrotic syndrome model rats, significantly improved blood biochemical indicators and renal function injury, and alleviated pathological changes in renal tissue and the foot process fusion of renal podocytes. Simultaneously, quercetin can reduce the expression of desmin and increase the expression of nephrin and synaptopodin. Quercetin can effectively treat nephrotic syndrome model rats induced by adriamycin, and it may play a protective role by stabilizing the normal structure and function of podocytes.

Key words: quercetin, nephrotic syndrome, adriamycin, urine protein, podocyte

CLC Number:

R965.1

SONG Zeyu, LI Zhenyuan, PAN Tao, MENG Xiangting, LI Song, DONG Hailun, FAN Huaying. Protective effect and mechanism of quercetin on adriamycin-induced nephrotic syndrome in rats[J].Shandong Science, 2023, 36(4): 61-68.

Figures/Tables 6

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References 22

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组别	24 h尿蛋白定量/mg
组别	第3 d	第7 d	第14 d	第21 d	第28 d
正常对照组	47.11±6.36	47.96±5.75	55.11±13.45	69.10±17.87	63.32±14.25
模型组	68.59±21.93	127.09±24.77^**	283.09±38.05^**	362.08±25.73^**	493.48±52.57^**
泼尼松组	74.04±21.93	129.08±24.77^**	195.56±38.05^##	226.77±25.73^##	235.98±52.57^##
25 mg/kg槲皮素组	80.24±18.42	127.74±25.41^**	246.89±26.43	336.82±17.50	409.22±49.13^##
50 mg/kg槲皮素组	75.02±17.71	124.49±19.16^**	213.84±37.87^##	271.87±47.38^##	310.37±51.92^##
100 mg/kg槲皮素组	73.32±20.80	121.39±11.09^**	203.41±40.17^##	224.42±54.35^##	228.40±46.20^##

组别	总蛋白质量浓度/ (g ·L^-1)	白蛋白质量浓度/ (g ·L^-1)	总胆固醇浓度/ (mmol ·L^-1)	甘油三酯浓度/ (mmol ·L^-1)
正常对照组	55.19±3.69	28.01±2.66	1.83±0.23	0.74±0.26
模型组	36.56±3.16^**	16.60±2.64^**	4.29±0.53^**	2.46±0.47^**
泼尼松组	51.86±2.47^##	24.96±1.84^##	2.73±0.68^##	1.25±0.34^##
25 mg/kg槲皮素组	39.59±4.99	15.56±2.98	4.40±0.47	2.44±0.39
50 mg/kg槲皮素组	51.47±2.78^##	21.98±3.25^#	3.34±0.48^##	1.91±0.23^#
100 mg/kg槲皮素组	53.00±6.94^##	26.10±1.81^##	2.41±0.84^##	1.63±0.25^##

组别	尿素氮浓度/(mmol ·L^-1)	血肌酐浓度/(μmol ·L^-1)
正常对照组	4.86±0.58	24.20±4.10
模型组	7.43±2.24^**	33.81±3.46^**
泼尼松组	5.12±1.23^#	26.18±3.44^##
25 mg/kg槲皮素组	7.18±1.41	31.41±5.63
50 mg/kg槲皮素组	6.32±1.16	30.31±3.26
100 mg/kg槲皮素组	5.08±0.82^##	24.94±4.66^##

Protective effect and mechanism of quercetin on adriamycin-induced nephrotic syndrome in rats

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