山东新分布药用植物碎米桠的DNA条形码分子鉴定

doi:10.3976/j.issn.1002-4026.2022.04.003

Abstract

Abstract:

DNA barcoding method was carried out to identify a species, which was discovered during field medicinal resource investigation in Shandong and difficult to identify using morphological characters. First, the total DNA was extracted from the samples. Then, the sequences of internal transcribed spacer region 2 and psbA-trnH were amplified and sequenced using polymerase chain reaction. Next, the obtained sequences were proofread and spliced using Chromasv, and the software MEGA 6.0 was used to analyze the data. Consequently, the studied species was identified as Isodon rubescen (Hemsl.) Hara using the DNA barcoding method. This study provides important evidence regarding the identification of a newly discovered species, Isodon rubescens, in Shandong and its medicinal resource development.

Key words: newly discovered species in Shandong, Isodon rubescens (Hemsl.) Hara, DNA barcoding, molecular identification

CLC Number:

R282.5

GE Jun-wei, LI Ping, ZHANG Tong-tong, GAO Fei, WANG Wen-yue, WANG Jun-nan, SUN Zhi-ying. Molecular identification of a newly discovered medicinal plant, Isodon rubescens, in Shandong using DNA barcoding[J].Shandong Science, 2022, 35(4): 15-20.

Figures/Tables 6

Table 1

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Table 3

Fig.3

References 23

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条形码名称	引物名称	引物序列(5'-3')
ITS2	ITS2F	ATGCGATACTTGGTGTGAAT
	ITS3R	GACGCTTCTCCAGACTACAAT
psbA-trnH	psbAF	GTTATGCATGAACGTAATGCTC
	trnHR	CGCGCATGGTGGATTCACAATCC

样品	ITS2差异位点
样品	21	22	32	42	43	71	202
wz1	C	A	C	-	-	T	-
wz2	*	*	*	*	*	*	*
wz3	*	*	*	*	*	*	*
smy-JQ389511	*	*	*	*	*	*	*
smy-KF032250	*	*	*	*	*	*	*
smy-KT285125	*	*	*	*	*	*	*
smy-JQ389509	*	*	*	*	*	*	*
nzx-KF032264	A	C	A	G	G	C	C
nzx-KF032265	A	C	A	G	G	C	C
nzx-KF032266	A	C	A	G	G	C	C

样品	psbA-trnH差异位点
样品	1	2	3	4	5	6	7	8	9	10	149	265	266	267	268	328	334	335	336	337	368	372	373	374	375	376	377	378	379	380	381	382	383	384	385	386	387	388	389	390	391
wz1	A	T	A	A	C	T	T	C	C	C	C	T	T	C	T	T	A	A	T	A	T	G	C	C	A	A	G	T	G	G	A	T	C	A	A	G	G	C	A	G	T
wz2	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*
wz3	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*
smy-KF513119	*	*	*	*	T	*	*	*	*	*	A	*	*	*	*	*	-	-	-	-	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*	*
smy-KF855542	-	-	-	-	-	-	-	-	-	-	A	*	*	*	*	*	*	*	*	*	*	*	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
nzx-FJ513099	*	*	*	*	*	*	*	*	*	*	A	-	-	-	-	G	*	*	*	*	G	A	*	*	G	*	*	*	*	A	*	*	G	*	*	*	*	G	G	A	*
nzx-KF032288	*	*	*	*	*	*	*	*	*	*	A	-	-	-	-	G	*	*	*	*	G	A	*	*	G	*	*	*	*	A	*	*	G	*	*	*	*	G	G	A	*

Molecular identification of a newly discovered medicinal plant, Isodon rubescens, in Shandong using DNA barcoding

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