Abstract: The species richness, diversity and composition of fungal community in wheat rhizosphere soil at different developmental stages with Trichoderma LTR-2 seed dressing agent were analyzed using Illumina MiSeq high-throughput sequencing platform. The results demonstrated that Trichoderma treatment of wheat seed increased the species richness of fungal community in the rhizosphere, and the community dominance increased significantly, while no obvious changes in the community uniformity were observed. In addition, the community composition was found similar with and without Trichoderma treatment. However, the proportion of various fungal groups in the rhizosphere changed significantly with Trichoderma treatment, among which Alternaria spp., Gibberella spp., and Fusarium spp. were decreased compared to non-seed dressing treatment. All the results provide a theoretical basis for better understanding the biocontrol mechanism of Trichoderma sp. LTR-2 against wheat soil-borne diseases.

Key words: Trichoderma sp., rhizosphere soil, high-throughput sequencing, soil fungi