Abstract: Glycine soja is the only wild relative which can crossfertilize cultivated soybean (Glycine max) and deliver fertile offspring.It has very strong salinity tolerance capability. Salt tolerance variant screening that is performed through sexual hybridization of wild and cultivated soybeans and tissue culture technology in vitro is an effective path to improve salt tolerance of soybean. We address induction and salinity tolerance of the calli of soybean mature embryo through the selection of five cultivated soybeans and wild soybean (Dongying 1). Results show that the survival rate of the wild soybean calliis remarkably higher than that of cultivated soybean calli, and that the descent range of the fresh weight of wild soybean calliis much lower than that of cultivated soybean calli for different concentrations NaCl stress.The superoxide dismutase and peroxida seactivities of the wild soybean calli are significantly higher than that of cultivated soybean calli.The malondialdehyde content of wild soybean calliis noticeably lower than that of cultivated soybean calli under NaCl stress.These indicate that the salinity tolerance of wild soybean calliis significantly superior to cultivated soybean calli, which provides a theoretical reference for the cultivation of new species salt tolerance soybean.

Key words: wild soybean, cultivated soybean, NaCl stress, calli