Abstract:

We constructed the method of isolating 6gingerol, 8gingerol and 6shogaol from dry rhizome of Zingiber officinale Rosc. by silica gel column chromatography and highspeed countercurrent chromatagraphy based on experiments. We extracted the samples of Zingiber officinale Rosc. by ethyl acetate ultrasonic extraction and then coarsely treated them by silica gel column chromatography. We further selected fraction A3 and A4 to be separated by highspeed countercurrent chromatography(HSCCC). The purity of the obtained single ingredient was detected by highperformance liquid chromatography(HPLC) and the structure of the obtained compound was identified by electrospray ionization(ESIMS) and 1HNMR. Results show that the method has such positives as simplicity, rapidity, highpurity products, large amount of preparation and highefficiency separation.

Key words: Zingiber officinale Rosc., highspeed countercurrent chromatagraphy, silica gel column, 6-gingerol, 8-gingerol, 6-shogaol