多菌灵降解菌的分离、鉴定及其降解特性研究

J4 ›› 2011, Vol. 24 ›› Issue (2): 28-34.

多菌灵降解菌的分离、鉴定及其降解特性研究

黄玉杰, 张新建, 任艳, 李纪顺, 张广志, 杨合同

山东省科学院中日友好生物技术研究中心，山东省应用微生物重点实验室，山东济南 250014

收稿日期:2011-01-05 出版日期:2011-04-20 发布日期:2011-04-20
作者简介:黄玉杰（1977-），女，助理研究员，硕士，主要从事土壤生物修复方面的研究
基金资助:
山东省自然基金项目（Y2007B57）；国际科技合作计划（2010DFA32330）

Isolation and identification of a carbendazim-degrading strain and its degrading characteristics

HUANG Yu-Jie, ZHANG Xin-Jian, REN Yan, LI Ji-Shun, ZHANG Guang-Zhi, YANG He-Tong

handong Provincial Key Laboratory of Applied Microbiology，Biotechnology Center, Shandong Academy of Sciences, Jinan 250014, China

Received:2011-01-05 Online:2011-04-20 Published:2011-04-20

摘要/Abstract

摘要：

从长期施用多菌灵的葡萄园土壤中分离纯化得到一株对多菌灵降解较好的菌株djl-11，经生理生化鉴定和16S rDNA序列比对分析，鉴定该菌株为红平红球菌（Rhodococcus erythropolis）。试验研究表明，添加少量氮源可促进菌株djl-11对多菌灵的降解作用。该菌能够以多菌灵为唯一碳源，在多菌灵浓度达到1000 μg/mL的无机盐培养基中，28℃摇床培养48 h降解率达到了99.15%。在pH值4~9之间均能降解多菌灵，最适温度在20~30℃。

关键词: 多菌灵, 生物降解, 红平红球菌, 降解特性

Abstract:

We obtained a strain dj1-11 from carbendazimcontaminated soil, which had better carbendazim degradation function. It was identified as Rhodococcus erythropolis through physiological and biochemical identification and contrast analysis of 16S rDNA sequences. Experimental results show that trace nitrogen source adding can improve carbendazim degradation. Carbendazim can serve as its unique carbon source and degradation rate can reach 99.15% under the condition of incubation in mineral salt medium of 1000 μg/mL carbendazim , temperature 28℃ and 48h shake bed.It can effectively degrade carbendazim at the pH value of 4~9 and temperature 20~30℃ (optimal degradation temperature).

Key words: carbendazim, biodegradation, Rhodococcus erythropolis, degrading characteristics

中图分类号:

X172

黄玉杰, 张新建, 任艳, 李纪顺, 张广志, 杨合同. 多菌灵降解菌的分离、鉴定及其降解特性研究[J]. J4, 2011, 24(2): 28-34.

HUANG Yu-Jie, ZHANG Xin-Jian, REN Yan, LI Ji-Shun, ZHANG Guang-Zhi, YANG He-Tong. Isolation and identification of a carbendazim-degrading strain and its degrading characteristics[J]. J4, 2011, 24(2): 28-34.