植酸酶工程菌产酶条件优化研究

doi:10.3976/j.issn.1002-4026.2018.04.013

山东科学 ›› 2018, Vol. 31 ›› Issue (4): 79-84.doi: 10.3976/j.issn.1002-4026.2018.04.013

植酸酶工程菌产酶条件优化研究

杨俊慧，公维丽，杨艳，孟庆军，史建国*

齐鲁工业大学（山东省科学院），山东省科学院生物研究所，山东省生物传感器重点实验室，山东济南 250014

收稿日期:2018-03-16 出版日期:2018-08-20 发布日期:2018-08-20
作者简介:杨俊慧（1969—），女，高级实验师，研究方向为生物传感器及生化分析。E-mail: swdz@sdas.org
基金资助:
山东省重点研发计划（2016ZDJS07A20）

A study of optimal fermentation conditions for phytase production by engineered strain

YANG Jun-hui, GONG Wei-li, YANG Yan, MENG Qing-jun, SHI Jian-guo*

Shandong Provincial Key Laboratory for Biosensors, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014，China

Received:2018-03-16 Online:2018-08-20 Published:2018-08-20

摘要/Abstract

摘要：

为研究影响植酸酶酵母工程菌的产酶因素，利用摇瓶发酵对毕赤酵母工程菌产植酸酶的最佳甲醇诱导量、诱导时间等因素进行实验分析。结果显示，残留甘油、甲醇浓度、诱导培养时间对植酸酶生产具有重要影响，其中甘油残留会使植酸酶分泌时间延迟，1.5% 甲醇具有最佳诱导效果，在第2天酶活可以达到约1 100 U/mL，低浓度甲醇诱导产酶缓慢积累，而高浓度甲醇对菌体产生毒害。该研究为进一步优化植酸酶工程菌高密度发酵工艺奠定了基础。

关键词: 毕赤酵母, 甲醇, 最佳培养条件, 植酸酶

Abstract:

To study the factors affecting phytase production by engineered Pichia pastoris, we explored the optimal fermentation conditions, such as methanolfedbatch concentration or induction time, with shake flask fermentation. The results demonstrated that a series of factors including residual glycerol, methanol concentration and induction time had significant effects on phytase production. Residual glycerol could delay the phytase secretion, 1.5% methanol had the best effect of induction with enzyme activity reaching about 1 100 U/mL in the second day, while low concentration of methanol could only slowly induce enzyme accumulation, and high concentration of methanol was harmful to engineered strain. This study lays a foundation for further optimization of high density fermentation conditions.

Key words: Pichia pastoris, optimal fermentation conditions, phytase, methanol

中图分类号:

Q936

杨俊慧，公维丽，杨艳，孟庆军，史建国. 植酸酶工程菌产酶条件优化研究[J]. 山东科学, 2018, 31(4): 79-84.

YANG Jun-hui, GONG Wei-li, YANG Yan, MENG Qing-jun, SHI Jian-guo. A study of optimal fermentation conditions for phytase production by engineered strain[J]. SHANDONG SCIENCE, 2018, 31(4): 79-84.

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植酸酶工程菌产酶条件优化研究

A study of optimal fermentation conditions for phytase production by engineered strain

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