关键词: 蚂蟥, 不同部位, 抗凝活性, 仿生提取, 盐水提取

Abstract: o study the anticoagulant activity from different parts of the Whitmania pigra Whitman, saline extraction and bionic extraction method were used respectively to extract the anticoagulant activity component in viscera, muscles, and hanging dry products of W. pigra. Thrombin time (TT), activated partial thromboplastin time (APTT), prothrombin time (PT), and antithrombin activity were selected as the activity indicators to evaluate the anticoagulant activities. It was found that when saline extraction was used, the TT and APTT of viscera were higher than those of muscles and hanging dry products, and there was no significant difference in the PT and antithrombin activity. When bionic extraction was adopted, in the TT and antithrombin activity experiment, the anticoagulant activity of viscera was lower than that of muscles and hanging dry products. In the APTT indicator measurement, viscera had strong anticoagulant activity compared with muscles and dried products, whereas there was no significant difference in the anticoagulant activity of various parts in the PT indicator measurement. Bionic extraction is more scientific than saline extraction because it stimulates the digestion process of W. pigra after oral administration. Therefore, different parts of W. pigra have different anticoagulant activities binding to different coagulation pathways, and the basis of anticoagulant materials is relatively dispersed.

Key words: Whitmania pigra Whitman, different parts, anticoagulant activity, bionic extraction, saline extraction