丹参花挥发油提取工艺优化及抗氧化活性研究

doi:10.3976/j.issn.1002-4026.2017.04.004

摘要/Abstract

摘要：

采用单因素法和L9(34)正交试验法，优化水蒸气蒸馏法提取丹参花挥发油的提取工艺，考察不同提取溶液、浸渍时间、蒸馏时间和料液比对丹参花挥发油收率的影响；应用DPPH法研究丹参花挥发油的抗氧化能力，并比较不同提取溶液所得挥发油的自由基清除能力。结果表明，影响丹参花挥发油收率的主要因素为蒸馏时间，其次为料液比、浸渍时间；最佳工艺条件为饱和氯化钠溶液、浸渍6 h、蒸馏8 h、料液比为1∶18，此条件下丹参花挥发油的收率为0.0935%。DPPH实验表明丹参花挥发油具有显著的抗氧化能力；在一定浓度范围内其抗氧化能力与挥发油浓度呈正相关性，蒸馏水、饱和氯化钠溶液、pH=1的硫酸水和pH=1的饱和氯化钠硫酸水4种提取液所得挥发油对DPPH的IC50值分别为1.07、1.03、1.45、1.40 mg/mL。通过验证实验表明，优化所得丹参花挥发油提取工艺稳定、易行。

关键词: 提取工艺, 抗氧化活性, 丹参花, DPPH, 挥发油

Abstract:

Through single factor experiment and L9(34) orthogonal test, the extraction process of volatile oil from flower of S. miltiorrhiza. by steam distillation was optimized and the effects of different extraction solutions, soaking time, extracting time, and solidtoliquid ratio on the yield of the volatile oil from the flower of S. miltiorrhiza. were investigated. The antioxidant capacity of volatile oil from S. miltiorrhiza flower was evaluated by DPPH method, and the free radical scavenging capacities of volatile oils from different extraction solutions were compared. The results showed that the primary factors affecting the yield of volatile oil from S. miltiorrhiza flower were distillation time, followed by solidliquid ratio and soaking time. The optimum extraction conditions were as follows: saturated sodium chloride solution, soaking 6 hours, extracting 8 hours, solidtosolvent ratio 1∶18(g/mL). Under this condition, the extraction rate of volatile oil from flower of S. miltiorrhiza was 0.0935%. The DPPH assays showed that volatile oil from flower of S. miltiorrhiza has significant antioxidant capacity. In a certain concentration range, antioxidant capacity was positively correlated with the concentration of volatile oil from flower of S. miltiorrhiza. In the DPPH assays, the IC50 values of volatile oil extracted by four kinds of extraction solution (distilled water, saturated sodium chloride solution, aqueous sulfuric acid of pH=1, saturated sodium chloride and aqueous sulfuric acid of pH=1) were 1.07, 1.03, 1.45 and 1.40 mg/mL, respectively. The results showed that the optimized extraction process of volatile oil from flower of S. miltiorrhiza was stable and practicable.

Key words: volatile oil, extraction process, antioxidant activity, flower of S. miltiorrhiza, DPPH

中图分类号:

R284.2

陈燕文，李玉娟，胡晶红，席晓志，刘谦，李佳，郝志*. 丹参花挥发油提取工艺优化及抗氧化活性研究[J]. 山东科学, 2017, 30(4): 19-25.

CHEN Yan-wen, LI Yu-juan, HU Jing-hong, XI Xiao-zhi, LIU Qian, LI Jia, HAO Zhi. Study on extraction technology of volatile oil from flower of Salvia miltiorrhiza Bge. and its antioxidant activity[J]. SHANDONG SCIENCE, 2017, 30(4): 19-25.

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