废弃烟叶中茄尼醇对照品的制备

J4 ›› 2011, Vol. 24 ›› Issue (3): 47-50.

废弃烟叶中茄尼醇对照品的制备

舒希凯^1,2, 林小静², 王岱杰², 刘代成¹, 耿岩玲², 刘静², 王晓^1,2

1.山东师范大学生命科学学院，山东济南 250014； 2.山东省科学院中药过程控制研究中心，山东省分析测试中心，山东济南 250014

收稿日期:2011-03-04 出版日期:2011-06-20 发布日期:2011-06-20
作者简介:舒希凯 (1988-)，男，硕士研究生，主要从事天然产物分离纯化与活性研究。
基金资助:
山东省科技攻关项目（2007GG20009005）；济南市高等院校自主创新计划（201004010）；济南市留学人员创业计划（20100409）

Preparation of reference material of solanesol from waste tobacco leaves

SHU Xi-Kai^1,2, LIN Xiao-Jing², WANG Dai-Jie², LIU Dai-Cheng¹, GENG Yan-Ling², LIU Jing², WANG Xiao^1,2

1.College of Life Science, Shandong Normal University, Jinan 250014, China; 2. Process Control Research Center of Traditional Chinese Medicine, Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China

Received:2011-03-04 Online:2011-06-20 Published:2011-06-20

摘要/Abstract

摘要：

建立从废弃烟叶中分离高纯度茄尼醇对照品的方法。首先采用石油醚萃取烟叶，动态皂化法皂化，硅胶柱层析进行粗分。制备型高效液相色谱采用甲醇∶乙醇=60∶40进行精制纯化。通过以上方法所得产物经ESI-MS、¹H-NMR和¹³C-NMR鉴定结构，高效液相色谱法测得纯度为99.7%，满足对照品的要求。本方法简便快捷，重现性好，所得产物纯度高，适合茄尼醇对照品的制备。

关键词: 废弃烟叶, 茄尼醇, 制备

Abstract:

This paper is to establish an approach to separate highpurity reference material of solanesol from tobacoo leaves. We initially obtained crude reference material of solanesol with petroleum ether extraction, dynamic saponification and silica gel chromatography. We then employed preparative highperformance liquid chromatography whose ingredient ratio was 60% methanol and 40% ethanol to refine and purify the acquired crude reference material of solanesol. The purified product whose structure was identified with ESI-MS,IR,¹H-NMR and ¹³C-NMR and whose purity of 99.7% was determined with HPLC satisfied the requirement of reference material. Such an approach is simple, rapid and reproductive and its product has quite high purity so it is suitable for the preparation of reference material of solanesol.

Key words: waste tobacoo leaves, solanesol, preparation

舒希凯, 林小静, 王岱杰, 刘代成, 耿岩玲, 刘静, 王晓. 废弃烟叶中茄尼醇对照品的制备[J]. J4, 2011, 24(3): 47-50.

SHU Xi-Kai, LIN Xiao-Jing, WANG Dai-Jie, LIU Dai-Cheng, GENG Yan-Ling, LIU Jing, WANG Xiao. Preparation of reference material of solanesol from waste tobacco leaves[J]. J4, 2011, 24(3): 47-50.

[1]	周鑫培, 陈伟, 何燕. 快速制备粉状活性焦烟气脱硫系统的数值研究[J]. 山东科学, 2021, 34(5): 86-96.
[2]	李明, 田明伟. 医用口罩的技术标准对比研究及前景展望[J]. 山东科学, 2020, 33(3): 28-34.
[3]	陈思彤, 尹艳艳, 尹敏, 薛富民, 孙齐磊 . Cs-Pb-Br体系钙钛矿材料光学性能研究进展[J]. 山东科学, 2020, 33(3): 75-81.
[4]	王玉真, 高爽, 孙越, 张丽, 薄福民, 封安杰, 李凌军. 菊苣酸脂质体制备工艺研究[J]. 山东科学, 2020, 33(1): 8-15.
[5]	田寒梅,侯延进,王建梅,李艳,王雪莹,王建春,许敏,王立秋. 基于ARM单片机全自动连续微球制备仪的研制[J]. 山东科学, 2017, 30(6): 94-98.
[6]	文蕾, 林云良, 高红梅, 李峰, 姜姣姣, 宋祥云, 付瑞明, 王岱杰. 山楂叶提取物中牡荆素、金丝桃苷、异槲皮苷对照品的制备[J]. 山东科学, 2017, 30(4): 13-18.
[7]	崔莉，陈静娴，朱姮，牟玉柱，林昌虎，张清海，王晓，王召平，王岱杰. 何首乌中顺式和反式-二苯乙烯苷对照品的制备[J]. 山东科学, 2015, 28(6): 6-10.
[8]	齐君，夏雪奎，贾爱荣，刘新，张绵松，刘昌衡. 二维高通量色谱分离制备海参来源真菌Epicoccum sp.的化学成分[J]. 山东科学, 2015, 28(4): 14-18.
[9]	陈立宗, 徐静, 吴守平, 田清波. 溶胶-凝胶法合成堇青石的研究进展[J]. 山东科学, 2011, 24(6): 30-35.