基于多技术联用的山东省不同产地金银花化学成分差异性分析

doi:10.3976/j.issn.1002-4026.2025170

山东科学

• 中药与天然活性产物 •

基于多技术联用的山东省不同产地金银花化学成分差异性分析

宁晓蓓¹，卢恒¹，王晓¹，康传志²，纪文华^1*

1 齐鲁工业大学（山东省科学院）山东省分析测试中心天然产物分离提取共性技术创新与应用山东省工程研究中心，山东济南 250014； 2 中国中医科学院中药资源中心，道地药材品质保障与资源持续利用全国重点实验室，北京 100700

收稿日期:2025-12-02 接受日期:2026-02-05 上线日期:2026-10-09
通信作者: 纪文华 E-mail:jwh519@163.com
作者简介:宁晓蓓（1999-），女，硕士研究生，研究方向：药物分析。E-mail：1366024369@qq.com
基金资助:
国家重点研发计划（2023YFC3503805）；中央本级重大增减支项目“名贵中药资源可持续利用能力建设目”（2060302)

Analysis of differences in the chemical composition of Lonicerae Japonicae Flos from different regions in Shandong Province based on combined multitechniques

NING Xiaobei¹, LU Heng¹, WANG Xiao¹, KANG Chuanzhi², JI Wenhua^1*

1. Shandong Engineering Research Center for Innovation and Application of General Technology for the Separation of Natural Products, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China) 2. State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China

Received:2025-12-02 Accepted:2026-02-05 Online:2026-10-09
Contact: JI Wenhua E-mail:jwh519@163.com

摘要/Abstract

摘要： 山东为中药金银花的核心产区，省内不同产地（尤其盐碱地）成分有差异，且单一检测技术难覆盖挥发性与非挥发性关键成分，制约产地溯源与品质评价。本研究以东营广饶、菏泽郓城、临沂沂南、临沂平邑4产地13批同源金银花为对象，采用顶空-气相色谱-离子迁移谱、顶空-固相微萃取-气相色谱-质谱、超高效液相色谱-四级杆飞行时间质谱三种分析技术，结合单因素方差分析、正交偏最小二乘法解析成分差异，共鉴定267种化合物，广饶独有5种萜类，与盐碱胁迫相关。离子迁移谱检出54种挥发性成分，其中醛、酮、醇和酯占87.0%；气相色谱-质谱筛选16种差异成分，其中9种为醛类；液相色谱-质谱筛选13种差异成分，正交偏最小二乘法模型稳定（R²_Y＞0.95，Q²＞0.89）。本研究多技术联用可为山东金银花品质评价与溯源提供依据。

关键词: 金银花, 产地差异, 离子迁移谱, 固相微萃取, 超高效液相色谱, 化学计量学

Abstract: Lonicerae Japonicae Flos (LJF) is a traditional Chinese medicine known for its heat-clearing and detoxifying properties, with Shandong Province being a major production area. However, studies on differences in chemical composition among different regions within Shandong (especially saline-alkali lands) are limited. Furthermore, a single analytical technique is insufficient to comprehensively cover volatile and nonvolatile key components, thereby limiting origin traceability and quality evaluation. In this study, 13 batches of genetically similar LJF samples from four regions in Shandong—Guangrao (Dongying), Yuncheng (Heze), Yinan (Linyi), and Pingyi (Linyi)—were analyzed using a combination of three techniques, i.e., headspace gas chromatography–ion mobility spectrometry (HS-GC-IMS), headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME-GC-MS), and ultra-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Chemometric methods including one-way analysis of variance and orthogonal partial least squares–discriminant analysis (OPLS-DA) were employed to analyze compositional differences. A total of 267 compounds were identified, including five terpenoids unique to Guangrao, which are associated with saline-alkali stress. HS-GC-IMS detected 54 volatile compounds, with aldehydes, ketones, alcohols, and esters accounting for 87.0% of the total. HS-SPME-GC-MS analysis identified 16 differential components, nine of which were aldehydes. UPLC-Q-TOF-MS analysis identified 13 differential components. The OPLS-DA model demonstrated good stability (R²_Y > 0.95, Q² > 0.89). This multitechnique approach provides a scientific basis for the quality evaluation and traceability of LJF in Shandong.

Key words: Lonicerae Japonicae Flos, different regions, ion mobility spectrometry, headspace solid-phase microextraction, ultra-performance liquid chromatography, chemometrics

中图分类号:

O657

宁晓蓓, 卢恒, 王晓, 康传志, 纪文华. 基于多技术联用的山东省不同产地金银花化学成分差异性分析[J]. 山东科学, 0, (): 1-.

NING Xiaobei, LU Heng, WANG Xiao, KANG Chuanzhi, JI Wenhua. Analysis of differences in the chemical composition of Lonicerae Japonicae Flos from different regions in Shandong Province based on combined multitechniques[J]. Shandong Science, 0, (): 1-.

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基于多技术联用的山东省不同产地金银花化学成分差异性分析

Analysis of differences in the chemical composition of Lonicerae Japonicae Flos from different regions in Shandong Province based on combined multitechniques

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