基于非靶向代谢组学技术的忍冬不同部位差异代谢物分析

doi:10.3976/j.issn.1002-4026.20240129

山东科学

• 中药与天然活性产物 •

基于非靶向代谢组学技术的忍冬不同部位差异代谢物分析

张绪鑫¹，张璐哲¹，林树乾³，赵增成³，孟兆青⁴，董晓弟⁴，曹桂云⁴，贺吉香^1*，王岱杰^2*

收稿日期：2024-11-08

基金项目：山东省重点研发计划（2023TZXD083），山东省重点研发计划重大科技创新工程项目（2021CXGC010511），齐鲁工业大学（山东省科学院）科教产融合试点工程（2024ZDZX14），泉城5150引才倍增计划

作者简介：张绪鑫（1998-），男，硕士研究生，研究方向为现代药物分析。E-mail：zzxinge0@qq.com

∗通信作者，贺吉香，女，教授，研究方向为现代药物分析。E-mail：987850250@qq.com

∗通信作者，王岱杰，男，研究员，研究方向为天然产物化学及标准样品研究。E-mail：wangdaijie@qlu.edu.cn

1.山东中医药大学药学院，山东济南 250355；2.齐鲁工业大学（山东省科学院），山东济南 250353；3.山东省农业科学院家禽研究所，山东济南 250100，4.山东宏济堂制药集团股份有限公司，山东济南 250102

收稿日期:2024-11-12 接受日期:2024-12-21 上线日期:2025-07-09
通信作者: 贺吉香/王岱杰 E-mail:987850250@qq.com/wangdaijie@qlu.edu.cn
作者简介:张绪鑫（1998-），男，硕士研究生，研究方向为现代药物分析。E-mail：zzxinge0@qq.com
基金资助:
山东省重点研发计划（2023TZXD083），山东省重点研发计划重大科技创新工程项目（2021CXGC010511），齐鲁工业大学（山东省科学院）科教产融合试点工程（2024ZDZX14），泉城5150引才倍增计划

Analysis of differential metabolites in different parts of Lonicera japonicaThunb.based on non-targeted metabolomics technology

ZHANG Xuxin¹，ZHANG Luzhe¹，LIN Shuqian³，ZHAO Zengcheng³，MENG Zhaoqing⁴，DONG Xiaodi⁴，CAO Guiyun⁴，HE Jixiang¹^*，WANG Daijie^2*

1.College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355,China; 2.Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353,China;3.Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Jinan 250100,China;4.Shandong Hongjitang Pharmaceutical Group. Co., Ltd., Jinan 250102,China

Received:2024-11-12 Accepted:2024-12-21 Published:2025-07-09
Contact: HE Jixiang/WANG Daijie E-mail:987850250@qq.com/wangdaijie@qlu.edu.cn

摘要/Abstract

摘要： 为研究忍冬不同部位的代谢物的差异性，采用基于超高效液相色谱串联质谱技术，对忍冬的茎、叶与花进行非靶向代谢组学研究。在忍冬不同部位样品中共鉴定到正离子模式代谢物773个，负离子模式代谢物572个。叶和花作比较共检测到553个差异代谢物；茎和花作比较共检测到471个差异代谢物，主要为酚酸类、氨基酸及其衍生物、黄酮以及异黄酮等化合物，差异代谢物相关的途径主要为苯丙烷生物合成、黄酮和黄酮醇生物合成、嘌呤代谢途径和花青素生物合成途径等。本研究分析了忍冬不同部位的优势化合物及参与的重要代谢途径，对比了茎和叶同药用部位花的差异性，为进一步开发利用忍冬植物资源提供了科学依据。

关键词: 非靶向代谢组学, 忍冬, 差异代谢物, 代谢通路

Abstract: To investigate the metabolic differences among different parts of Lonicera japonica Thunb.,a nontargeted metabolomics study was conducted on its stems, leaves, and flowers using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).Intotal,773 metabolites in positive-ion mode and 572 and 572 metabolites in negative-ion mode were identified in samples from different parts of L. japonica Thunb.; 553 differential metabolites were detected by comparing leaves and flowers; and 471 differential metabolites were detected by comparing stems and flowers, mainly phenolic acids, amino acids and their derivatives, flavonoids, and isoflavones. The pathways related to differential metabolites mainly include phenylpropanoid biosynthesis, flavonoid and flavonol biosynthesis, purine metabolism, and anthocyanin biosynthesis. This study analyzed the predominant compounds and important metabolic pathways involved in different parts ofL. japonica Thunb.,i.e.,the stems,leaves, and flowers,comparing them with medically used flowers. The findings provides a scientific basis for the further development and utilization ofL. japonica Thunb.resources.

Key words: nontargeted metabolomics, Lonicera japonica Thunb., differential metabolites, metabolic pathway

中图分类号:

R284.1

张绪鑫, 张璐哲, 林树乾, 赵增成, 孟兆青, 董晓弟, 曹桂云, 贺吉香 , 王岱杰. 基于非靶向代谢组学技术的忍冬不同部位差异代谢物分析[J]. 山东科学, 0, (): 1-.

ZHANG Xuxin, ZHANG Luzhe, LIN Shuqian, ZHAO Zengcheng, MENG Zhaoqing, DONG Xiaodi, CAO Guiyun, HE Jixiang, WANG Daijie. Analysis of differential metabolites in different parts of Lonicera japonicaThunb.based on non-targeted metabolomics technology[J]. Shandong Science, 0, (): 1-.

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基于非靶向代谢组学技术的忍冬不同部位差异代谢物分析

Analysis of differential metabolites in different parts of Lonicera japonicaThunb.based on non-targeted metabolomics technology

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