关键词: 秦艽, 标准汤剂, 指纹图谱, 经典名方, 质量传递性

Abstract: High-performance liquid chromatography (HPLC) fingerprint spectra were created for 15 batches of Radix Gentianae Macrophyllae medicinal herb and standard decoctions of medicinal slices.Acetonitrile and 0.1% acetic acid were used as the mobile phase,and 254 nm was maintained as the detection wavelength.Simultaneously,in combination with content determination and extract yielding rate,mass transfer was examined with respect to the medicinal herb and standard decoctions of medicinal slices.The fingerprint spectra of the medicinal herb and the standard decoctions of medicinal slices shared high similarity with the respective control spectra.With gentiopicroside as the reference peak,five common peaks were confirmed and five chromatographic peaks were identified.The average extract yielding rate of the 15 batches of both the medicinal herb and the standard decoctions of medicinal slices was 20.43% and 19.71%,respectively.The average transfer rate of gentiopicroside and loganic acid from the medicinal herbs to the slices was 80.56% and 80.98% respectively;no discrete data were obtained.The results showed that the quality control and transferability of the herbs and the slices were investigated using the pattern (with overall control over the material group) of combining the fingerprint spectra with extract yielding rate and content determination indicators;this can serve as a reference for research concerning classic prescriptions and traditional Chinese medicine formula granules.

Key words: Radix Gentianae Macrophyllae, standard decoctions of medicinal slices, fingerprint, classic prescriptions, mass transfer