酶联免疫法测定水产品中己烯雌酚残留量

doi:10.3976/j.issn.1002-4026.2019.03.012

山东科学 ›› 2019, Vol. 32 ›› Issue (3): 80-84.doi: 10.3976/j.issn.1002-4026.2019.03.012

酶联免疫法测定水产品中己烯雌酚残留量

张瑞凌¹，许亦真¹，刘建萍²，李福伟¹，张冲¹

1.齐鲁工业大学（山东省科学院），山东省分析测试中心，山东济南 250014；2.山东蓝城分析测试有限公司，山东济南 250014

收稿日期:2018-06-08 出版日期:2019-06-20 发布日期:2019-06-01
作者简介:张瑞凌（1983—），女，实验师，研究方向为食品检测和环境检测。E-mail：zhangrl@sdatc.com.cn

Determination of diethylstillbestrol residues in aquatic products by enzyme linked immunosorbent assay

ZHANG Rui-ling¹, XU Yi-zhen¹, LIU Jian-ping², LI Fu-wei¹, ZHANG Chong¹

1.Shandong Analysis and Test Center, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250014, China; 2.Shandong Bluetown Analysis and Teat Co., LTD, Jinan 250014, China

Received:2018-06-08 Online:2019-06-20 Published:2019-06-01

摘要/Abstract

摘要： 采用酶联免疫法测定水产品中残留己烯雌酚的含量，并采用液相色谱-质谱联用法验证阳性结果。实验结果表明，该方法对水产品中己烯雌酚的检出限为0.35 μg/kg，回收率达77%～91%，具有良好的灵敏度和准确度。

关键词: 酶联免疫, 己烯雌酚, 水产品

Abstract: The content of diethylstilbestrol(DES)residues in aquatic products was determined by enzyme linked immunosorbent assay(ELISA),and the positive results were verified by liquid chromatography-mass spectrometry (LC-MS).The final test results show that the detection limit of DES in aquatic products is 0.35 μg/kg and the recovery rate is 77%～91%. This method has good sensitivity and accuracy.

Key words: Enzyme linked immunosorbent assay (ELISA), diethylstilbestrol (DES), aquatic products

中图分类号:

O657.63

张瑞凌, 许亦真, 刘建萍, 李福伟, 张冲. 酶联免疫法测定水产品中己烯雌酚残留量[J]. 山东科学, 2019, 32(3): 80-84.

ZHANG Rui-ling, XU Yi-zhen, LIU Jian-ping, LI Fu-wei, ZHANG Chong. Determination of diethylstillbestrol residues in aquatic products by enzyme linked immunosorbent assay[J]. Shandong Science, 2019, 32(3): 80-84.

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酶联免疫法测定水产品中己烯雌酚残留量

Determination of diethylstillbestrol residues in aquatic products by enzyme linked immunosorbent assay

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