山东科学 ›› 2016, Vol. 29 ›› Issue (1): 21-24.doi: 10.3976/j.issn.1002-4026.2016.01.004

• 中药与天然活性产物 • 上一篇    下一篇

入侵植物春飞蓬的生物检定与化学成分初探

王辉,赵睿,黄陆军,张世尧,王琦,徐凌川   

  1. 山东中医药大学,山东 济南 250355
  • 收稿日期:2015-07-25 出版日期:2016-02-20 发布日期:2016-02-20
  • 通信作者: 徐凌川(1962-),男,教授,硕士生导师,研究方向为生药中药质量控制、资源开发利用及药用真菌研究与应用。 E-mail:xulingchuan518@sina.com
  • 作者简介:王辉(1990-),女,硕士研究生,研究方向为中药、生药资源及质量控制与资源开发利用。Email:m15216440087_1@163.com。
  • 基金资助:

    国家公益性行业专项基金(201407002)

Biological identification and chemical constituents analysis of invasive plant Erigeron philadelphicus L.

WANG Hui, ZHAO Rui, HUANG Lujun, ZHANG Shiyao, WANG Qi, XU Lingchuan   

  1. Shandong University of Traditional Chinese Medicine, Jinan 250355, China
  • Received:2015-07-25 Online:2016-02-20 Published:2016-02-20

摘要:

为了对入侵植物春飞蓬进行生物检定和化学成分初步研究,采用基原鉴别、显微鉴别、分子鉴别、紫外光谱及高效液相色谱等方法进行实验研究,并与近似植物一年蓬进行花粉粒和DNA序列比较。结果表明,春飞蓬粉末中的主要显微特征为具三孔沟的花粉粒、不定式气孔、多细胞非腺毛、冠毛以及导管等;春飞蓬与一年蓬的花粉粒形态差异显著;二者的DNA序列区别明显;春飞蓬总黄酮的含量为059%,未测出灯盏花乙素。

关键词: 春飞蓬, 显微鉴别, 总黄酮, 资源利用

Abstract:

We use origin identification, microscope identification, molecule identification, UV and HPLC to preliminarily analyze chemical constituents of the alien invasive plant Erigeron philadelphicus L. We also compare pollen grain and DNA sequence between Erigeron philadelphicus L. and its similar plant Erigeron annuus(L.) Pers.Results show that predominant microscopic characters of  E. Philadelphicus L. powder are pollen grain, infinitive stomata, multicell nonglandular hairs,pappus,vessel, etc. Form distinction of their pollen grains is significant.The difference of their DNA sequence is obvious. Total flavonoids content of Erigeron philadelphicus L. is 0.59% and we do not find its breviscapine.

Key words: Erigeron philadelphicus L., total flavones, resource utilization, microscopic identification

中图分类号: 

  • R284.1