不同价态的阴离子在DNA凝聚效应中的抑制作用

doi:10.3976/j.issn.1002-4026.2020.05.015

山东科学 ›› 2020, Vol. 33 ›› Issue (5): 119-126.doi: 10.3976/j.issn.1002-4026.2020.05.015

不同价态的阴离子在DNA凝聚效应中的抑制作用

李聪聪，杨光参，王艳伟*

温州大学数理学院，浙江温州 325035

收稿日期:2020-05-07 出版日期:2020-10-08 发布日期:2020-09-27
通信作者: 王艳伟，女，副教授。 E-mail: wangyw@wzu.cn E-mail:1369869166@qq.com
作者简介:李聪聪（1994—），男，硕士研究生，研究方向为生命现象中的凝聚态物理。Tel：15158721830,E-mail:1369869166@qq.com
基金资助:
国家自然科学基金(11574232,11274245);浙江省自然科学基金(LY17A040006)

Inhibition of DNA condensation by anions of various valencies

LI Cong-cong, YANG Guang-can, WANG Yan-wei*

School of Mathematics and Physics, Wenzhou University, Wenzhou 325035, China

Received:2020-05-07 Online:2020-10-08 Published:2020-09-27

摘要/Abstract

摘要： 为了定量地研究阴离子对DNA凝聚和电荷中和过程的抑制作用，利用动态光散射和原子力显微镜研究了不同价态阴离子对阳离子介导的DNA凝聚效应的影响。在动态光散射实验中，阴离子可以使阳离子作用后的DNA的电泳迁移率变小，即对DNA的电荷中和有抑制作用，而且阴离子价态越高，对电泳迁移率的抑制作用越强。改变阳离子种类但控制相同的阳离子浓度，发现阴离子对DNA的电荷中和有同样的抑制作用及趋势，同时用原子力显微镜观察DNA的凝聚形态变化也得出一致的结果。在相同阳离子浓度下，高价阴离子相对低价态阴离子，对DNA凝聚的抑制作用更强，使DNA的凝聚形态变得更加松散，进一步证实了阴离子对阳离子导致的DNA凝聚有减弱作用。

关键词: DNA凝聚, 电荷逆转, 阴离子, 阳离子, 抑制作用

Abstract: To quantitatively study the inhibitory effect of anions on DNA condensation and charge neutralization, the effect of anions of various valencies on cation-mediated DNA condensation was studied using dynamic light scattering and atomic force microscopy (ATM). Dynamic light scattering experiments showed that anions reduced the electrophoretic mobility of DNA in the presence of cations, indicating that charge neutralization of DNA was inhibited. The higher valence of anions, the stronger was the inhibition of electrophoretic mobility. Different anions exerted the same inhibitory effect and trend on DNA charge neutralization at the same concentration of various cations. Meanwhile, the morphological changes in DNA condensation as observed by AFM led to the same conclusion. At the same cation concentration, highvalent cations showed more potent inhibition on DNA condensation than lowvalent cations, which make the DNA aggregation morphology more loose. These observations further confirmed that anions have a weakening effect on cationmediated DNA condensation.

Key words: DNA condensation, charge inversion, anion, cation, inhibitory effect

中图分类号:

李聪聪, 杨光参, 王艳伟. 不同价态的阴离子在DNA凝聚效应中的抑制作用[J]. 山东科学, 2020, 33(5): 119-126.

LI Cong-cong, YANG Guang-can, WANG Yan-wei. Inhibition of DNA condensation by anions of various valencies[J]. Shandong Science, 2020, 33(5): 119-126.

参考文献 19

1	SURANA S, SHENOY A R., KRISHNAN Y. Designing DNA nanodevices for compatibility with the immune system of higher organisms[J]. Nature Nanotechnology, 2015, 10 (9): 741-747. doi: 10.1038/nnano.2015.180
2	MANNING G S.The molecular theory of polyelectrolyte solutions with applications to the electrosttic properties of polynucleotides[J].Quarterly Reviews of Biophysics, 1978, 11 (2): 179-246. doi: 10.1017/S0033583500002031
3	LUO Z X, WANG Y W, LI S H,et al. DNA phase transition in charge neutralization and comformation induced by trivalent-hydrolysed metal ions[J].Polymers, 2018,10(4):394. doi: 10.3390/polym10040394
4	CHAI A H, RAN S Y, ZHANG D, et al. Processes of DNA condensation induced by multivalent cations: approximate annealing experiments and molecular dynamics simulations[J]. Chinese Physics B, 2013, 22 (9): 648-655. doi: 10.1088/1674-1056/22/9/09870
5	DEROUCHEY J, HOOVER B, RAU D C. A comparison of DNA compaction by arginine and lysine peptides: a physical basis for arginine rich protamines[J]. Biochemistry, 2013,52(17):3000-3009. doi: 10.1021/bi4001408
6	芦洁坪, 王艳伟, 杨光参. 组氨酸对DNA电荷逆转和凝聚的影响[J]. 山东科学,2018,31(4): 126-132,138. doi: 10.3976/j.issn.1002-4026.2018.04.019
7	VOGELE K, LIST J, PARDATSCHER G, et al. Self-Assembled active plasmonic waveguide with a peptide-based thermomechanical switch[J].Acs Nano.2016,10(12):11377-11384. doi: 10.1021/acsnano.6b06635
8	KATZ A M, TOLOKH I S, PABIT S A, et al. Spermine condenses DNA, but not RNA duplexes[J]. Biophys J,2017, 112 (1): 22-30. doi: 10.1016/j.bpj.2016.11.018
9	WANG Y W, RAN S Y, MAN B Y, et al. DNA condensations on mica surfaces induced collaboratively by alcohol and hexammine cobalt[J]. Colloids and Surfaces B Biointerfaces,2011,83 (1): 61-68. doi: 10.1016/j.colsurfb.2010.10.040
10	ZINCHENKO A, TSUMOTO K, MURATA S, et al. Crowding by anionic nanoparticles causes DNA double-strand instability and compaction[J]. Journal of Physical Chemistry B,2014, 118 (5): 1256-1262. doi: 10.1021/jp4107712
11	LI G Y, GUAN R L, JI L N, et al. DNA condensation induced by metal complexes[J]. Coordination Chemistry Reviews, 2014,281: 100-113. doi: 10.1016/j.ccr.2014.09.005
12	SAITO T, IWAKI T, YOSHIKAWA K. Small anion with higher valency retards the compaction of DNA in the presence of multivalent cation[J]. Biophysical Journal, 2009,96 (3): 1068-1075. doi: 10.1016/j.bpj.2008.11.008
13	YOSHIKAWA K, KIDOAKI S, TAKAHASHI M, et al. Marked discreteness on the coil-globule transition of single duplex DNA[J].Chemical Physics, 2010, 100 (6): 876-880. doi: 10.1002/bbpc.19961000631
14	YOSHIKAWA Y, YOSHIKAWA K. Diaminoalkanes with an odd number of carbon atoms induce compaction of a single double-stranded DNA chain[J]. Febs Letters, 1995, 361 (2-3): 277-281. doi: 10.1016/0014-5793(95)00190-K
15	DUC N V, NGUYEN T T, DUC N H. Multivalent co-ions reduce DNA-DNA like-charge attraction and enhance DNA overcharging by mutivalent counterions.[J]. J Biol Phys ,2017,43:185–195. doi: 10.1007/s10867-017-9443-x
16	龚红玲.DNA凝聚过程中温度及离子浓度效应实验研究 [D].贵阳：贵州大学,2017.
17	蒋杨伟.半刚性高分子链熔体中的熵相互作用及离子相互作用引起的DNA凝聚/解凝转变 [D].杭州：浙江大学, 2017.
18	VAN M W, UNDERWOOD S M. Dynamic-light-scattering study of glasses of hard colloidal spheres[J]. Physical Review E Statistical Physics Plasmas Fluids Related Interdisciplinary Topics, 1993, 47 (1): 248-261. doi: 10.1103/PhyRevE.47.248
19	NEUMAN K C, NAGY A. Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy[J].Nature Methods, 2008, 5 (6): 491-505. doi: 10.1002/pros.20587

[1]	芦洁坪，王艳伟，杨光参. 组氨酸对DNA电荷逆转和凝聚的影响[J]. 山东科学, 2018, 31(4): 126-132.
[2]	郭子龙,王艳伟,杨光参. 铁离子引起的DNA电荷逆转和凝聚[J]. 山东科学, 2016, 29(4): 93-98.

不同价态的阴离子在DNA凝聚效应中的抑制作用

Inhibition of DNA condensation by anions of various valencies

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献 19

相关文章 2

Metrics

本文评价

推荐阅读 0