基于网络药理学研究厚朴-杏仁治疗哮喘的潜在作用机制

doi:10.3976/j.issn.1002-4026.2020.04.005

摘要/Abstract

摘要： 本研究采用网络药理学方法分析厚朴-杏仁配伍治疗哮喘的潜在作用机制。通过BATMAN-TCM数据库检索厚朴、杏仁的中药化学成分及靶标信息，PALM-IST数据库搜索疾病靶标信息，string数据库构建靶标的蛋白质相互作用网络，利用Cytoscape v3.5.1对药物靶标相互作用进行可视化分析，DAVID数据库和Cytoscape v3.5.1的ClueGO+CluePedia插件对药物关键靶标进行GO生物功能富集分析和KEGG信号通路富集分析。共检索到厚朴-杏仁化合物51个，靶标435个，哮喘疾病相关靶标415个，进而分析得到药物治疗疾病关键靶标14个，包括肿瘤坏死因子、过氧化氢酶、白介素13、β2肾上腺素能受体等；GO基因功能分析主要涉及应激反应调控、先天性免疫反应调控、防御反应、急性炎症反应、刺激反应、凝血止血调节、自我内稳定、活性氧代谢过程等生物过程；KEGG分析主要参与NF-κB、IL-17、FcεRI、Toll受体及FoxO等信号通路。厚朴-杏仁能通过多个靶点、多种途径、多条通路对哮喘的气道慢性炎症、气道重塑和气道高反应起潜在治疗作用。

关键词: 网络药理学, 厚朴-杏仁, 治疗, 哮喘, 作用机制

Abstract: In the present study, network pharmacological methods were used to analyze the potential mechanism of Magnolia Officinalis Cortex-Armeniacae Semen Amarum combination in the treatment of asthma. We searched the chemical constituents and related information of the Traditional Chinese Medicine Magnolia Officinalis Cortex-Armeniacae Semen Amarum combination using the BATMAN-TCM database, disease-related information using the PALM-IST database, and construct target protein interaction network based on the String database. Cytoscape v3.5.1 software was used for the visual analysis of drugtarget interaction. The DAVID database and ClueGO + CluePedia plug-in for Cytoscape v3.5.1 were used for GO biofunctional enrichment analysis and KEGG signal pathway enrichment analysis of the key drug targets. In the present study, 51 compounds and 435 targets of Magnolia Officinalis Cortex-Armeniacae Semen Amarum combination were found. There were 415 targets related to asthma.Further, 14 key targets for drug treatment of the disease were obtained, such as tumor necrosis factor, catalase, interleukin-13, and β2-adrenergic receptor. GO gene analysis mainly involves biological processes such as stress response regulation, innate immune response regulation, defense response, acute inflammatory response, response to stimulus, blood coagulation and hemostasis regulation, self-internal stability, and reactive oxygen species metabolic process. KEGG analysis was primarily involved in NF-κB, IL-17, FcεRI, Toll receptor, FoxO, and other signaling pathways. In conclusion, we observed that the Magnolia Officinalis Cortex-Armeniacae Semen Amarum combination can play potential therapeutic roles in chronic airway inflammation, remodeling, and hyperresponsiveness in asthma via multiple targets, approaches, and pathways.

Key words: network pharmacology, Magnolia Officinalis Cortex-Armeniacae Semen Amarum combination, treatment, asthma, mechanismu

中图分类号:

R285.5

李焕敏, 李新民, 孙丹, 韩耀巍, 孙婷, 律扬, 陈鸿祥. 基于网络药理学研究厚朴-杏仁治疗哮喘的潜在作用机制[J]. 山东科学, 2020, 33(4): 24-33.

LI Huan-min, LI Xin-min, SUN Dan, HAN Yao-wei, SUN Ting, LU Yang, CHEN Hong-xiang. Potential mechanism of the Magnolia Officinalis Cortex-Armeniacae Semen Amarum combination in the treatment of asthma based on network pharmacology[J]. Shandong Science, 2020, 33(4): 24-33.

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