山东科学 ›› 2018, Vol. 31 ›› Issue (1): 39-.doi: 10.3976/j.issn.1002-4026.2018.01.007

• 农业微生物 • 上一篇    下一篇

越南伯克氏菌及其组合生防菌防治黄瓜根结线虫田间试验

王贻莲1 ,王珍伟 ,刘宝军1 , 潘明2  ,李纪顺1*   

  1. 1.齐鲁工业大学(山东省科学院),山东省科学院生态研究所,山东省应用微生物重点实验室,山东 济南 250103;2.济南五福生物技术有限公司,山东 济南250103
  • 收稿日期:2017-10-13 出版日期:2018-02-20 发布日期:2018-02-20
  • 作者简介:王贻莲(1978—),女,副研究员,研究方向为应用微生物。 E-mail:yilianwang@163.com,Tel:13573791095
  • 基金资助:

    山东省重点研发计划(2016GNC113009); 山东省科学院枣庄市产业技术研发联合基金(201503);山东省科学院-单县人民政府产学研合作引导基金(201501)

Field trial of Burkholderia vietnamiensis and its composite microbial flora on cucumber rootknot nematode

WANG Yi-Lian1,WANG Zhen-Wei2, LIU Bao-Jun1, PAN Ming2, LI Ji-Shun1*   

  1. 1. Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology( Shandong Academy of Sciences), Jinan 250103, China; 2.Jinan Wufu Biotechnology Co., Ltd, Jinan 250103, China
  • Received:2017-10-13 Online:2018-02-20 Published:2018-02-20

摘要:

为提高越南伯克氏菌及其组合生防菌对蔬菜根结线虫病的田间防治效果,以黄瓜根结线虫为研究对象,采用在整地时添加营养基质、生物菌肥、微生物菌剂,移栽时再穴施微生物菌剂的处理方式,研究越南伯克氏B418及其与蜡样芽胞杆菌BCJB01、木霉菌LTR2和T11W的组合菌剂的配套应用技术。结果表明,整地时添加营养基质,可显著提高B418菌剂对黄瓜根结线虫的田间防治效果;组合菌剂处理中,在整地时同时添加生物菌肥和营养基质的处理对黄瓜根结线虫的防治效果好于单加营养基质或生物菌肥处理;B418单一菌剂+营养基质处理优于组合菌剂+营养基质处理, 防治效果为90.05%。

关键词: 木霉, 生物防治, 越南伯克氏菌, 蜡样芽胞杆菌, 根结线虫

Abstract:

In order to improve the control effect of Burkholderia vietnamiensis and its composite microbial flora on rootknot nematode in vegetables, taking the cucumber rootknot nematode as the research object, we adopted the way of adding nutrient matrix, biobacteria fertilizer and microbial antimicrobial agents when cultivating land, and fertilizing microbial antimicrobial agents when transplanting, carried out research for application technology on Burkholderia vietnamiensis B418 and its combinations with Bacillus cereus BCJB01, Trichoderma spp.LTR-2 and T11-W. The results showed that the addition of nutrient substrate when cultivating land could significantly improve the control effect of B418 to rootknot nematode. In the treatment of composite microbial flora, the treatment of cucumber rootknot nematode by adding bio-bacteria fertilizer and nutrient matrix at the same time when cultivating land was better than that of single nutrition matrix or bio-fertilizer. B418 single agent add nutrient matrix treatment was superior to its composite microbial flora add nutrition matrix treatment, whose field control effectiveness was 90.05%.

Key words: Burkholderia vietnamiensis , Trichoderma spp., biological control, Bacillus cereus, Meloidogyne spp.

中图分类号: 

  • S476