J4 ›› 2013, Vol. 26 ›› Issue (2): 56-60.doi: 10.3976/j.issn.1002-4026.2013.02.011

• 中药与天然活性产物专栏 • 上一篇    下一篇

化学发光法检测不同干燥方法对金银花抗氧化活性和化学成分的影响

王梅,刘峰,林昌虎,张清海,张永清,刘建华,王晓   

  1. 1. 山东师范大学生命科学学院,山东 济南 250014; 2. 山东省分析测试中心,山东 济南 250014; 3.贵州省理化测试分析研究中心,贵州省科学院,贵州 贵阳 550002; 4.山东中医药大学,山东 济南 250355
  • 收稿日期:2012-11-28 出版日期:2013-04-20 发布日期:2013-04-20
  • 通信作者: 王晓,研究员,研究方向为天然产物化学。 E-mail:wangxiao2008@yahoo.cn
  • 作者简介:王梅(1987-),女,硕士研究生,研究方向为天然产物。Email:lovewangmei521@126.com
  • 基金资助:

    国家“十二五”科技支撑计划(2011BAI06B01);贵州省中药现代化科技产业研究开发专项(黔科合重大专项字[2012]6010号)

Impacts of different drying methods on antioxidant activity and chemical ingredients of honeysuckle by chemiluminescence detection

 WANG Mei, LIU Feng, LIN Chang-Hu, ZHANG Qing-Hai, ZHANG Yong-Qing, LIU Jian-Hua, WANG Xiao   

  1. 1.College of Life Sciences, Shandong Normal University, Jinan 250014, China; 2. Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China; 3.Guizhou Research Center of Physical Test and Chemical Analysis,Guizhou Academy of Sciences,Guiyang 550002,China; 4.Shandong University of Traditional Chinese Medicine,Jinan 250355,China
  • Received:2012-11-28 Online:2013-04-20 Published:2013-04-20

摘要:

研究不同干燥方法(微波、鼓风干燥、阴干) 对金银花的抗氧化活性和总酚、总黄酮质量分数的影响。利用鲁米诺化学发光体系,以抗坏血酸为阳性对照,以超氧阴离子(O-2)、双氧水(H2O2)和羟基自由基(HO·)清除为指标,评价3种不同干燥方法对金银花的抗氧化活性的影响,并对总酚和总黄酮的质量分数进行了测定。实验结果表明:金银花具有较强的抗氧化活性;不同的干燥方法对其活性物质的质量分数以及抗氧化活性的强度均有较强的影响,其中70 ℃微波干燥10 min条件下,金银花总酚和总黄酮的质量分数最高,分别为30.40±2.75 mg/g、61.24±7.62 mg/g,并且其抗氧化活性最强,3种指标的IC-50分别为金银花原提取液浓度的9.44%、0.029%、0.12%。而自然阴干4 d的条件下,金银花的总酚和总黄酮的质量分数最低,分别为10.22±0.81 mg/g、17.50±2.28 mg/g,并且抗氧化活性最弱,3种指标的IC-50分别为金银花原提取液浓度的11.97%、0.53%、0.37%。

关键词: 金银花, 干燥方法, 抗氧化能力, 总酚, 总黄酮

Abstract:

We addressed the impacts of different drying methods (microwavedrying, hotair drying and shadedrying) on the antioxidant activity and chemical ingredient of honeysuckle. We also assessed the impacts of the three drying methods on the antioxidant activity of honeysuckle and determined the content of total phenolic and total flavonoid with Luminol chemiluminescence, and superoxide anion, hydrogen peroxide and hydroxyl radical scavenging as indices. Experimental results show that honeysuckle has remarkable antioxidant activity. The three different drying methods all exhibit significant impacts on the antioxidant activity and the content of active components. Antioxidant activity of total phenolic and total flavonoid is the strongest, and their content is the highest, 30.40±2.75 mg/g and 61.24±7.62 mg/g under the conditions of microwave drying at 70 ℃ for 10 min. IC-50 of the three indices is 9.44%, 0.029% and 0.12%. Antioxidant activity of total phenolic and total flavonoid is the weakest and their contents are the lowest, 10.22±0.81mg/g and 17.50±2.28 mg/g under the condition of shadedrying for 4 days. IC-50 of the three indices is 11.97%, 0.53% and 0.37% of the original extract concentration.

Key words: honeysuckle, drying method, antioxidant activity, total phenolic, total flavonoid

中图分类号: 

  • O657.1