J4 ›› 2012, Vol. 25 ›› Issue (4): 27-31.doi: 10.3976/j.issn.1002-4026.2012.04.007

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鲜艾叶与干艾叶挥发性成分的HS-SPME-GC-MS分析

王丽1,2,赵先恩1,王晓1,耿岩玲1,刘建华1,刘伟1   

  1. 1.山东省分析测试中心,山东 济南 250014; 2.山东农业大学食品科学与工程学院,山东 泰安 271018
  • 收稿日期:2012-04-08 出版日期:2012-08-20 发布日期:2012-08-20
  • 通信作者: 刘伟,研究方向为中药过程控制与中药化学。 E-mail:wxjn1998@126.com
  • 作者简介:王丽(1987-),硕士研究生,研究方向为天然产物分离纯化。Email:guest108800@163.com
  • 基金资助:

    山东省科技攻关项目(2010GSF10287)

HS-SPME-GC-MS analysis of volatile components in fresh and dried leaves of  Artemisia argyi

 WANG Li1,2, ZHAO Xian-En1, WANG Xiao1, GENG Yan-Ling1, LIU Jian-Hua1, LIU Wei1   

  1. 1.Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China; 2.School of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China
  • Received:2012-04-08 Online:2012-08-20 Published:2012-08-20

摘要:

       采用顶空固相微萃取-气相色谱质谱联用(HS-SPME-GC-MS)技术分析比较鲜艾叶与干艾叶的挥发性成分。考察了不同萃取头和萃取温度对艾叶挥发性成分的萃取效果,以选用65 μm PDMS/DVB SPME萃取头和60 ℃水浴加热时萃取效果最好;采用峰面积归一化法测定各成分的质量分数,共鉴定出58种挥发性成分,主要为烯烃类、芳香类和醇类物质,占总峰面积90%以上。鲜艾叶与干艾叶均含有莰烯、1-辛烯-3-醇、α-水芹烯、邻聚伞花素、桉油精、石竹烯等,此外又各自具有特有成分,表明艾叶在干制后其挥发性成分发生变化。

关键词: 鲜艾叶, 干艾叶, 挥发性成分, 顶空固相微萃取, 气质联用

Abstract:

        We analyzed volatile components in fresh and dried leaves of Artemisia argyi Levl. et Vant. with headspace solid phase microextraction ( SPME) and GC-MS. We also investigated the impacts of different SPME fibers and extraction temperature on the extracts. Results demonstrate that 65 μm PDMS /DVB fibre and temperature of 60 ℃ exhibit good extraction effect.We employed peak area normalization method to determine the volatile components. Fifty-eight compounds are identified in the fresh and dried leaves of Artemisia argyi, which include alkanes,alkenes and alcohols,and whose area exceeds 90% of the total peak area. Results also show that fresh and dried leaves of Artemisia argyi not only contain identical volatile compounds such as Camphene, 1-Octen-3-ol, alpha Phellandrene, Benzene, 1-methyl-2 (1-methylethyl) Eucalyptol and Caryophyllene, but also contain their own unique volatile compounds. This shows that their volatile  components  change after dryness.

Key words: fresh leaves of , Artemisia argyi, dried leaves of Artemisia argyi, volatile components, headspace solid phase microextraction (HS-SPME), GC-MS

中图分类号: 

  • O652.62