碳氮元素数量比对典型耐盐微生物增殖影响

doi:10.3976/j.issn.1002-4026.20240128

摘要/Abstract

摘要：

贝莱斯芽孢杆菌(Bacillus velezensis)、苏云金芽孢杆菌(Bacillus thuringiensis)和耐寒短杆菌(Brevibacterium frigoritolerans)是3种典型的植物耐盐根际促生细菌(ST-PGPR),具有缓解植物盐胁迫等作用。为优化这3种菌株的生长与增殖营养条件,采用单因素实验方法研究了不同碳氮元素数量比(碳氮比,n_C/n_N)对其生长和增殖的影响。结果显示,3种菌株在碳氮比为3.25~6.00范围内均可生长繁殖,且不同碳氮比对其增殖有显著影响,其中对贝莱斯芽孢杆菌繁殖的影响最显著,苏云金芽孢杆菌次之,耐寒短杆菌的影响最小。当碳氮比为4:1时,贝莱斯芽孢杆菌增殖数量最多,活菌数量可达6.2×10⁸CFU/mL;苏云金芽孢杆菌在碳氮比为4∶1时增殖数量最多,活菌数量为5.1×10⁸CFU/mL;耐寒短杆菌在碳氮比为5∶1时增殖数量最多,活菌数量为3.5×10⁸CFU/mL。综上,贝莱斯芽孢杆菌和苏云金芽孢杆菌增殖最佳的碳氮比为4:1,耐寒短杆菌增殖最佳的碳氮比为5:1。

关键词: 耐盐微生物, 碳氮比, 培养基优化, 微生物生物量

Abstract:

Bacillus velezensis, Bacillus thuringiensis and Brevibacterium frigoritolerans are three typical salt-tolerant plant growth-promoting rhizobacteria (ST-PGPR) known for their ability to alleviate salt stress in plants. To optimize the nutritional conditions for the growth and proliferation of these strains, experiment of single factor method was used to investigate the effects of different carbon-to-nitrogen ratios (n_C/n_N) on their development. The results showed that all three strains could grow and reproduce in a medium with a n_C/n_N of 3.25~6.00. However, the n_C/n_N had varying effects on their proliferation, i.e., the most significant impact was observed on the proliferation of velezensis, followed by thuringiensis and frigoritolerans. When the n_C/n_N was 4:1, velezensis exhibited the highest proliferation, with a viable bacterial count of 6.2 × 10⁸ CFU/mL. Similarly, thuringiensis achieved its highest proliferation at a n_C/n_N of 4:1, with a viable bacterial count of 5.1 × 10⁸ CFU/mL. Conversely, frigoritolerans achieved its highest proliferation at a n_C/n_N of 5:1, with a viable bacterial count of 3.5 × 10⁸ CFU/mL. In conclusion, the optimal n_C/n_N for the proliferation of velezensis and thuringiensis is 4:1, whereas that for frigoritolerans is 5:1.

Key words: salt-tolerant microorganisms, carbon-to-nitrogen ratios, medium optimization, microbial biomass

中图分类号:

Q939.9

王晨, 卢雅欣, 李晓勇, 张嘉祎, 许乐天, 冯庆. 碳氮元素数量比对典型耐盐微生物增殖影响[J]. 山东科学, 2025, 38(2): 73-79.

WANG Chen, LU Yaxin, LI Xiaoyong, ZHANG Jiayi, XU Letian, FENG Qing. Effects of carbon-to-nitrogen ratio on the proliferation of typical salt-tolerant microorganisms[J]. Shandong Science, 2025, 38(2): 73-79.

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实验组	m(葡萄糖)/g	m(蛋白胨)/g	碳氮比	m(KH₂PO₄)/g	m(MgSO₄·7H₂O)/g
1	0	1.0	3.25:1	0.2	0.5
2	0.2	1.0	4:1	0.2	0.5
3	1.2	1.0	7:1	0.2	0.5
4	2.1	1.0	10:1	0.2	0.5

碳氮比	生物量(OD₆₀₀)	菌种数量×10^-8/(CFU·mL^-1)
3.25:1	2.165	3.2
4:1	2.284	4.5
7:1	2.028	1.3
10:1	2.005	0.3

实验组	m(葡萄糖)/g	m(蛋白胨)/g	碳氮比	m(KH₂PO₄)/g	m(MgSO₄·7H₂O)/g
1	0	1.0	3.25:1	0.2	0.5
2	0.2	1.0	4:1	0.2	0.5
3	0.5	1.0	5:1	0.2	0.5
4	0.9	1.0	6:1	0.2	0.5

碳氮比 (n_C/n_N)	生物量(OD)			菌种数量×10^-8/(CFU·mL^-1)
碳氮比 (n_C/n_N)	苏云金芽孢杆菌	贝莱斯芽孢杆菌	耐寒短杆菌	苏云金芽孢杆菌	贝莱斯芽孢杆菌	耐寒短杆菌
3.25:1	2.235	2.322	2.117	3.4	3.6	1.7
4:1	2.292	2.523	2.187	5.1	6.2	2.4
5:1	2.183	2.307	2.236	2.6	4.7	3.5
6:1	2.030	2.273	2.191	1.5	3.8	2.6