HPLC-DAD法同时测定三种来源丹参饮片中水溶性和脂溶性成分

doi:10.3976/j.issn.1002-4026.2019.06.004

山东科学 ›› 2019, Vol. 32 ›› Issue (6): 20-25.doi: 10.3976/j.issn.1002-4026.2019.06.004

HPLC-DAD法同时测定三种来源丹参饮片中水溶性和脂溶性成分

张敏敏，赵志国，刘伟，崔莉，王岱杰，王晓，赵恒强^*

齐鲁工业大学(山东省科学院）山东省分析测试中心山东省中药质量控制技术重点实验室，山东济南 250014

收稿日期:2019-08-19 出版日期:2019-12-20 发布日期:2019-12-06
通信作者: 赵恒强（1980—），博士，副研究员，研究方向为中药分析与质量控制研究。Tel: 0531-82605319，E-mail:hqzhao2007@163.com
作者简介:张敏敏（1990—），硕士，研究方向为中药分析与质量控制。 E-mail: zhangminminff@163.com
基金资助:
山东省自然科学基金（ZR2017LH071,ZR2017LH069）；现代农业产业技术体系建设专项资金（CARS-21）；“十三五”重大专项生态种植项目（SQ2017YFC170295）；“十三五”重点研发计划（2017YFC1702701）：山东省泰山学者岗位资助项目（郭兰萍）；山东省科学院先导专项（郭兰萍）

Simultaneous detection of water- and fat-soluble components of Danshen decoction slices of three origins using HPLC-DAD

ZHANG Min-min,ZHAO Zhi-guo, LIU Wei, CUI Li, WANG Dai-jie, WANG Xiao, ZHAO Heng-qiang^*

Shandong Provincial Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center,Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China

Received:2019-08-19 Online:2019-12-20 Published:2019-12-06

摘要/Abstract

摘要： 测定不同来源丹参饮片中水溶性和脂溶性成分含量，为丹参的质量评价和合理利用提供数据支持。发展了HPLC-DAD同时测定丹参中的6种水溶性和脂溶性成分的方法，利用该方法对来源于山东、四川和云南的丹参饮片样品进行含量测定，结合聚类分析进行不同来源丹参饮片的差异性对比。结果表明：该方法简单、快速，可以实现丹参中不同极性成分的同时提取、分析；不同来源的丹参饮片中水溶性和脂溶性成分含量均存在一定的差异，来源于四川产区的丹参饮片中各成分含量最高，来源于山东产区的丹参饮片酮类成分含量优于云南产区，采用活性成分含量结合聚类分析可以实现其来源区分；不同来源的丹参饮片中各活性成分含量存在一定的差异。研究结果为丹参饮片的质量评价和合理利用提供了方法和数据支持。

关键词: 丹参, 多指标定量, 聚类分析, 产地差异性

Abstract: This study determined the contents of water- and fat-soluble components of Danshen of different origins,and provided supporting data for the qualitative evaluation and rational utilization of Danshen decoction slices. We developed a method for the simultaneous determination of six water- and fat-soluble components of Danshen using HPLC-DAD . A method combined with cluster analysis was applied for differential comparison of Danshen decoction slice samples of three different origins: Shandong, Sichuan, and Yunnan. The results showed that this method could simultaneously, easily, and rapidly extract different polar components of Danshen decoction slices. There were some differences in the contents of water- and fat-soluble components in different batches of Danshen decoction slices. Among these, the content of each component in Danshen decoction slices from Sichuan was the highest, and the content of tanshinones from Shandong was better than that from Yunnan. The combination of active ingredient content and cluster analysis could achieve the conclusion of the production area.In summary, there are differences in the contents of components of Danshen decoction slices of different origins. This study provides methods and supporting data for the qualitative evaluation and rational utilization of Danshen decoction slices.

Key words: Danshen, multi-index quantification, cluster analysis, origin difference

中图分类号:

R917

张敏敏, 赵志国, 刘伟, 崔莉, 王岱杰, 王晓, 赵恒强. HPLC-DAD法同时测定三种来源丹参饮片中水溶性和脂溶性成分[J]. 山东科学, 2019, 32(6): 20-25.

ZHANG Min-min, ZHAO Zhi-guo, LIU Wei, CUI Li, WANG Dai-jie, WANG Xiao, ZHAO Heng-qiang. Simultaneous detection of water- and fat-soluble components of Danshen decoction slices of three origins using HPLC-DAD[J]. Shandong Science, 2019, 32(6): 20-25.

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HPLC-DAD法同时测定三种来源丹参饮片中水溶性和脂溶性成分

Simultaneous detection of water- and fat-soluble components of Danshen decoction slices of three origins using HPLC-DAD

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