海水叶绿素a传感器荧光性能评估方法

doi:10.3976/j.issn.1002-4026.2025003

山东科学 ›› 2025, Vol. 38 ›› Issue (3): 84-89.doi: 10.3976/j.issn.1002-4026.2025003

海水叶绿素a传感器荧光性能评估方法

王爱军(), 王聪, 石超英, 赵虹

国家海洋标准计量中心自然资源部海洋计量检测技术创新中心,天津 300112

收稿日期:2025-01-09 出版日期:2025-06-20 发布日期:2025-06-26
作者简介:王爱军(1980—),女,硕士,正高级工程师,研究方向为海洋仪器计量校准方法研究。E-mail:wangaj@ncosm.org.cn
基金资助:
国家重点研发计划项目(2022YFC2804004)

A standardized method for evaluating the fluorescence performance of seawater chlorophyll-a sensors

WANG Aijun(), WANG Cong, SHI Chaoying, ZHAO Hong

Technology Innovation Center for Marine Metrology and Instruments Test, MNR, National Center of Ocean Standards and Metrology, Tianjin 300112, China

Received:2025-01-09 Online:2025-06-20 Published:2025-06-26

摘要/Abstract

摘要：

海水叶绿素a传感器广泛应用于海洋现场生态监测中,用来观察海水中叶绿素a质量浓度的变化,但测量结果容易漂移。为了获得高质量的海水叶绿素a观测数据,对海水叶绿素a传感器性能评估方法进行了研究。利用荧光素钠标准品作为参考标准物质,从响应线性范围、准确性、精密度、稳定性等4个方面研究了海水叶绿素a传感器的性能评估方法,结果表明,传感器的荧光信号与荧光溶液的质量浓度呈正相关关系,利用其拟合后的标准曲线计算得到传感器的示值误差、实验标准偏差,通过控制相关系数可以确定传感器的线性响应范围,利用同一传感器在不同时期的重复性实验数据考察了传感器的稳定性,从计量角度实现了传感器的特性评估。在响应线性范围(0~200 μg/L)内,传感器的误差最大不超过2.00 μg/L,相对实验标准偏差不超过0.20%,在2021—2022年传感器比较稳定,其中试验过程中移液精度保持不变是保证结果准确的关键因素。

关键词: 海水叶绿素a传感器, 荧光标准物质, 评估方法, 移液精度

Abstract:

Seawater chlorophyll-a sensors are essential tools for marine ecological monitoring, enabling the detection of spatial and temporal variations in chlorophyll-a concentration. However, these sensors are susceptible to measurement drift, which can compromise data reliability. This study proposes a metrological approach for evaluating sensor performance using fluorescein sodium as a reference standard. Sensor performance was assessed in terms of linear response range, accuracy, precision, and stability. Results indicated a strong positive correlation between fluorescence intensity and fluorescein concentration. Based on the fitted calibration curve, indication error and standard deviation were calculated. The linear response range was determined by controlling the correlation coefficient, whereas stability was assessed through repeated measurements over different time periods. Within the linear range of 0 to 200 μg/L, the maximum measurement error was ≤2.00 μg/L, and the relative standard deviation was <0.20%. The sensor exhibited consistent performance from 2021 to 2022. Maintaining consistent pipetting accuracy was identified as a critical factor for ensuring measurement reliability.

Key words: seawater chlorophyll-a senaor, fluorescence standard material, evaluation method, pipette accuracy

中图分类号:

P716.6

王爱军, 王聪, 石超英, 赵虹. 海水叶绿素a传感器荧光性能评估方法[J]. 山东科学, 2025, 38(3): 84-89.

WANG Aijun, WANG Cong, SHI Chaoying, ZHAO Hong. A standardized method for evaluating the fluorescence performance of seawater chlorophyll-a sensors[J]. Shandong Science, 2025, 38(3): 84-89.

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砝码质量 /mg	测量次数										标准偏差 /mg	最小称样量/mg
砝码质量 /mg	1	2	3	4	5	6	7	8	9	10	标准偏差 /mg	最小称样量/mg
10.00	9.98	9.98	9.98	9.98	10.00	9.98	10.00	9.98	10.00	9.98	0.009 7	0.001 9
20.00	20.01	20.00	19.99	19.99	19.98	19.98	20.02	20.03	20.02	20.03	0.019 6	0.002 0
40.00	39.99	40.01	40.01	40.00	40.00	39.99	39.99	39.99	39.99	39.99	0.008 4	0.000 4

溶液质量浓度/(μg·L^-1)	传感器荧光信号平均值	传感器测量值/(μg·L^-1)	传感器示值误差/(μg·L^-1)
20.00	0.227 7	21.29	1.29
120.00	1.126 7	121.85	1.85
170.00	1.548 3	169.01	-0.99

海水叶绿素a传感器荧光性能评估方法

A standardized method for evaluating the fluorescence performance of seawater chlorophyll-a sensors

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