关键词: 五味保肝丸, 细胞色素P450, CYP2E1

Abstract:

To investigate the protective effect and the mechanism of Wuwei Baoganwan(WBW) on alcohol-induced liver injury in mice, fifty healthy male KM mice were randomly divided into five groups: normal control (NC) group, model control (MC) group, high-,medium-,and low-dose WBW group, 10 mice in each group. To those in NC and MC group, 20 g/L CMC-Na Solution was given; those in high-,medium-,and low-dose WBW group were treated with WBW suspl. of 0.5, 0.25, 0.125 g/kg, respectively. The medication in all groups was given through intragastric administration, once a day for ten consecutive days. After 2 hours of administration every day, the mice of MC group and WBW groups were orally given 56% alcohol of 12 mL/kg to establish alcoholinduced liver injury model. And to those in the NC group, equal dose of normal saline was given. The activity of ALT, AST, the content of CYP450 in liver microsome and the activity of CYP2E1 were respectively measured. Compared with the MC group, the ALT and AST levels were notably inhibited to increase in the three doses of WBW groups. The CYP450 content in the liver microsome were obviously increased. The activity of CYP2E1 was significantly decreased. Among them, there was significant difference between the middle and high dose of WBW groups（P<0.05）.WBW shows protective effect on alcohol-induced liver injury in mice which might be related to its increasing the content of CYP450 and reducing the activity of CYP2E1.

Key words: Wuwei Baoganwan, CYP2E1, CYP450