拮抗性多粘类芽孢杆菌PB-2及其制剂

doi:10.3976/j.issn.1002-4026.2014.03.006

J4 ›› 2014, Vol. 27 ›› Issue (3): 30-33.doi: 10.3976/j.issn.1002-4026.2014.03.006

拮抗性多粘类芽孢杆菌PB-2及其制剂

扈进冬¹，吴远征¹，李纪顺¹，魏艳丽¹，陈泉¹，杨合同1,2

1.山东省科学院生物技术研究中心, 山东省应用微生物重点实验室，山东济南 250014； 2.山东理工大学生命科学学院，山东淄博 255049

收稿日期:2013-11-26 出版日期:2014-06-20 发布日期:2014-06-20
作者简介:扈进冬（1977-），男，硕士，高级工程师，研究方向为农业微生物。Email：hujd@sdas.org
基金资助:
公益性行业（农业）科研专项经费（201303025）

The control effects of antagonistic Paenibacillus polymyxa PB-2 and its preparation against grape downy mildew

HU Jin-Dong¹, WU Yuan-Zheng¹, LI Ji-Shun¹, WEI Yan-Li¹, CHEN Quan¹, YANG He-Tong^1,2

1. Shandong Provincial Key Laboratory of Applied Microorganism, Biotechnology Center,Shandong Academy of Sciences,Jinan 250014, China; 2. School of Life Sciences,Shandong University of Technology, Zibo 255049, China

Received:2013-11-26 Online:2014-06-20 Published:2014-06-20

摘要/Abstract

摘要：

采用叶盘漂浮法室内测定拮抗性多粘类芽孢杆菌PB2对葡萄霜霉病的防治效果，结果表明，PB-2含菌量为10⁸ cfu/mL的发酵液和过滤除菌的无菌发酵滤液对葡萄霜霉病菌均具有较好的抑制作用，抑制率分别达到了77.21%和60.24%。制备了PB-2可湿性粉剂，田间试验表明，PB-2可湿性粉剂稀释50倍和100倍液对葡萄霜霉病1周后相对防效分别为46.91％和36.99％，2周后分别为46.44％和42.10%。该研究表明PB-2可湿性粉剂对葡萄霜霉病具有良好的田间应用前景。

关键词: 葡萄霜霉病, 多粘类芽孢杆菌, 田间防效

Abstract:

We employed floating leaf disc test to determine the control effects of antagonistic Paenibacillus polymyxa PB-2 against grape downy mildew. Results show that when PB-2 is 10⁸ cfu/mL,the fermentation broth and aseptic fermentation filtrate both have better inhibitory effects on grape downy mildew. Their inhibitory rates are 77.21% and 60.24%. We also prepared wettable powder (WP) of PB-2. Field tests indicate that the relative control effects of 50 and 100 times dilution PB-2 WP against grape downy mildew are 46.91% and 36.99% after 1 week and 46.44% and 42.10% after 2 weeks. This demonstrates that PB-2 WP has better field application prospect for controlling grape downy mildew.

Key words: grape downy mildew, Paenibacillus polymyxa, field evaluation

中图分类号:

S182

扈进冬，吴远征，李纪顺，魏艳丽，陈泉，杨合同. 拮抗性多粘类芽孢杆菌PB-2及其制剂[J]. J4, 2014, 27(3): 30-33.

HU Jin-Dong, WU Yuan-Zheng, LI Ji-Shun, WEI Yan-Li, CHEN Quan, YANG He-Tong. The control effects of antagonistic Paenibacillus polymyxa PB-2 and its preparation against grape downy mildew[J]. J4, 2014, 27(3): 30-33.

拮抗性多粘类芽孢杆菌PB-2及其制剂

The control effects of antagonistic Paenibacillus polymyxa PB-2 and its preparation against grape downy mildew

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