地衣共生菌分离培养的新方法

J4 ›› 2011, Vol. 24 ›› Issue (1): 33-35.

地衣共生菌分离培养的新方法

邱振鲁, 王海英, 赵遵田

山东师范大学生命科学学院，山东济南 250014

出版日期:2011-02-20 发布日期:2011-02-20
基金资助:
济南市科技局（200914006）

New method for isolation and culture of lichenized fungi

QIU Zhen-Lu, WANG Hai-Ying, ZHAO Zun-Tian

College of Life Sciences, Shandong Normal University, Jinan 250014, China

Online:2011-02-20 Published:2011-02-20

摘要/Abstract

摘要：

介绍了一种分离培养地衣共生菌和内生菌的新方法。该方法直接挑取地衣体髓层菌丝进行培养，PDA培养基25℃培养14 d后观察，发现共生菌菌落生长率80.43%，而杂菌污染率为7.14%，具有简单、高效和污染率低的优点。

关键词: 地衣共生菌, 地衣内生菌, 分离培养

Abstract:

This paper presented a new method for isolation and culture of lichenized fungi and lichen endophyte fungi. We directly selected medulla mycelium for cultivation with PDA nutrition medium. The growth rate of the community of lichenized fungi was 80.43%, while the contamination rate was only 7.14% under the conditions of nutrition medium of 25℃ and 14 days later. The superiority of this method was therefore simplicity, highefficiency and low contamination rate.

Key words: lichenized fungi, lichen endophyte fungi, isolation and culture

中图分类号:

Q949.34

邱振鲁, 王海英, 赵遵田. 地衣共生菌分离培养的新方法[J]. J4, 2011, 24(1): 33-35.

QIU Zhen-Lu, WANG Hai-Ying, ZHAO Zun-Tian. New method for isolation and culture of lichenized fungi[J]. J4, 2011, 24(1): 33-35.