新月菱形藻的紫外诱变及优化培养

J4 ›› 2011, Vol. 24 ›› Issue (1): 22-27.

新月菱形藻的紫外诱变及优化培养

张新建, 任艳, 黄玉杰, 宋莉璐, 李红梅, 赵晓燕, 张广志, 杨合同

山东省科学院中日友好生物技术研究中心，山东省应用微生物重点实验室，山东济南 250014

出版日期:2011-02-20 发布日期:2011-02-20
基金资助:
山东省科技发展计划项目（2008GG10002025）

UVInduction and culture optimization for Nitzschia closterium

ZHANG Xin-Jian, REN Yan, HUANG Yu-Jie, SONG Li-Lu, LI Hong-Mei, ZHAO Xiao-Yan, ZHANG Guang-Zhi, YANG He-Tong

Shandong Provincial Key Laboratory of Applied Microbiology, Biotechnology Cener, Shandong Academy of Sciences, Jinan 250014, China

Online:2011-02-20 Published:2011-02-20

摘要/Abstract

摘要：

本实验室通过紫外诱变得到一株富含油脂的新月菱形藻BC6，在实验室条件下研究了不同温度、光照强度、pH值、N源含量、NaCl含量等培养条件对BC6生长的影响。研究结果表明：最适培养温度在20 ℃，pH值为7.5，最适光照强度为54 μE/(m2·s)，NaNO3的最适添加量为105 mg/L，NaCl最适添加量为27 g/L。在优化培养条件下，最高细胞密度可达1.69×106 /mL，比优化前增大了2.2倍。

关键词: 新月菱形藻, 紫外诱变, 培养条件, 优化培养

Abstract:

We obtained a strain of oilrich [WTBX]Nitzschia closterium[WTBZ] (BC6) through UVinduction. We further investigated the effects of different culture conditions such as temperature, irradiance, pH value, salinity and nitrogen concentration on its growth. The OD680 and growth rate were determined every two days. Experimental results show that the optimum culture conditions are temperature of 20 ℃, pH value of 7.5, irradiance of 54 μmol·m^-2·s^-1, NaNO₃ concentration of 105 mg·L^-1and NaCl concentration of 27 g·L^-1. Its highest cell density can reach 1.69×10⁶/mL, 2.2 times higher than that of nonoptimization, under these optimal conditions.

Key words: Nitzschia closterium, UVinduction, culture conditions, culture optimization

中图分类号:

Q949.2

张新建, 任艳, 黄玉杰, 宋莉璐, 李红梅, 赵晓燕, 张广志, 杨合同. 新月菱形藻的紫外诱变及优化培养[J]. J4, 2011, 24(1): 22-27.

ZHANG Xin-Jian, REN Yan, HUANG Yu-Jie, SONG Li-Lu, LI Hong-Mei, ZHAO Xiao-Yan, ZHANG Guang-Zhi, YANG He-Tong. UVInduction and culture optimization for Nitzschia closterium[J]. J4, 2011, 24(1): 22-27.