B418对连作小油菜产量及根际土壤氮循环基因和cbbL固碳基因的影响

doi:10.3976/j.issn.1002-4026.2025076

山东科学

• 农业微生物 •

B418对连作小油菜产量及根际土壤氮循环基因和cbbL固碳基因的影响

王贻莲¹，扈进冬¹，黄鼎立²，徐维生³，杨合同¹，李纪顺^1*，何春雨⁴

1.齐鲁工业大学(山东省科学院) 生态研究所黄河流域轻工有机污染物低碳处置与资源化利用协同创新中心，山东济南 250103；2.威海益丰农业科技有限公司，山东威海264200 ；3.山东省沂水县诸葛镇林业站，山东临沂276422；4.甘肃中医药大学药学院，甘肃兰州 730000

收稿日期:2025-07-21 接受日期:2025-11-03 上线日期:2025-12-23
通信作者: 李纪顺 E-mail:sdyewu2@163.com
作者简介:王贻莲（1978-），女，副研究员，硕士，研究方向为设施蔬菜田退化土壤的生物修复。 E-mail:yilianwang@163.com
基金资助:
齐鲁工业大学（山东省科学院）科教产融合试点工程重大创新类项目：设施蔬菜退化土壤微生态修复与病虫害绿色防控技术体系构建与应用（2025ZDZX18）；齐鲁工业大学（山东省科学院）产学研协同创新基金项目:叶菜类连作障碍土壤微生物修复技术（2020-CXY11）；威海市政府-山东省科学院产学研协同创新基金项目(2020GC05)

Effects of B418 on the yield of continuously cropped Rape (Brassica chinensis L.) and on the nitrogen-cycling and carbon-fixation gene cbbL in rhizosphere soils

WANG Yilian¹, HU Jindong¹, HUANG Dingli², XU Weisheng³, YANG Hetong¹, LI Jishun^1*, HE Chunyu⁴

1. The Collaborative Innovation Center of Yellow River Basin for Low-carbon Disposal and Resource Utilization of Organic Pollutants in Light Industry, Ecology Institute of Shandong Academy of Sciences, Qilu University of Technology (Shandong Academy of Sciences),Jinan,250103; 2. Weihai Yifeng Agricultural Science and Technology Co. , Ltd. Weihai, 264200；3. Forestry station of Zhuge Town, Yishui County, Shandong Province, Linyi 276422；4. Pharmacy College of Gansu University of Chinese Medicine, Lanzhou 730000

Received:2025-07-21 Accepted:2025-11-03 Online:2025-12-23
Contact: LI Jishun E-mail:sdyewu2@163.com

摘要/Abstract

摘要： 为探明越南伯克霍尔德氏菌B418缓解连作障碍土壤对小油菜生产的限制作用，采用盆栽试验，研究了外源添加B418对连作小油菜的出苗，存活及其根际土壤氮循环相关功能基因和固碳基因的影响，并探讨功能基因与产量的相关性，以期为连作蔬菜增产提供科学依据。结果表明：次生盐渍化轻度障碍土壤Ⅰ（pH4.3，EC 1361μS·cm^-1）和次生盐渍化重度障碍土壤Ⅱ（pH4.6，EC 2200μS·cm^-1）中，B418处理均能提高小油菜出苗率、存活率并增加产量，促使根际土壤固碳基因（cbbL）的绝度丰度增加。土壤Ⅰ中，B418处理固氮酶 nifH和氨单加氧酶amoA基因丰度提高，氧化亚氮还原酶nosZ基因丰度降低；土壤Ⅱ中，B418处理nifH基因丰度提高，amoA、亚硝酸盐还原酶基因nirK、nirS和nosZ基因丰度降低，且处理间差异显著，表明外源添加B418，可抑制连作小油菜土壤根际硝化作用和反硝化作用。相关性分析结果显示，产量与cbbL和nifH基因丰度呈正相关，与nirK基因丰度呈负相关，与amoA、nirS和nosZ基因丰度呈显著负相关；amoA与nosZ基因丰度呈极显著正相关，表明连作小油菜产量的提高与根际微生物的固碳、氮能力及抑制硝化作用和反硝化作用密切相关。

关键词: 越南伯克霍尔德氏菌, 连作障碍, 小油菜, 氮循环相关基因, 固碳基因丰度

Abstract: To clarify how B418 alleviates the limitations imposed by continuous-cropping obstacle soils on rape production, a plot experiment was conducted to investigate the effects of exogenously applied B418 on the emergence, survival, and yield of rape, as well as on nitrogen transformation-related genes and carbon-fixation genes in the rhizosphere soil of continuously cropped rape (Brassica napus L.). Correlations between functional gene abundance and yield were also analyzed to provide a scientific basis for increasing the yield of continuously cropped vegetables. The results showed that in mildly secondary salinized obstacle soil I (pH 4.3, EC 1361 μs·cm^?1) and severely secondary salinized obstacle soil II (pH 4.6, EC 2200 μs·cm^?1), B418 significantly increased the emergence rate, survival rate, and yield of rape and increased the absolute abundance of the carbon-fixation gene cbbL in rhizosphere soil. In soil I, B418 treatment increased the abundance of the nitrogenase gene nifH and the ammonia monooxygenase gene amoA, but decreased the abundance of the nitrous oxide reductase gene nosZ. In soil II, B418 treatment increased the abundance of nifH while decreasing the abundance of amoA, the nitrite reductase genes nirK and nirS, and nosZ, with significant differences between treatments. These results indicate that exogenously added B418 can suppress rhizosphere nitrification and denitrification in continuously cropped rape. Correlation analysis showed that yield was positively correlated with the abundance of cbbL and nifH, negatively correlated with nirK, and significantly negatively correlated with the abundance of amoA, nirS, and nosZ. The abundance of amoA was extremely significantly positively correlated with that of nosZ, indicating that yield improvement in continuously cropped rape is closely related to rhizosphere microbial carbon-fixation and nitrogen-cycling capacities, as well as to the suppression of nitrification and denitrification.

Key words: Burkholderia vietnamiensisB418, continuous cropping obstacles, Brassica chinensisL, nitrogen-cycling genes, carbon-fixation gene abundance

中图分类号:

S636.9

王贻莲, 扈进冬 , 黄鼎立, 徐维生, 杨合同 , 李纪顺, 何春雨. B418对连作小油菜产量及根际土壤氮循环基因和cbbL固碳基因的影响[J]. 山东科学, 0, (): 1-.

WANG Yilian, HU Jindong, HUANG Dingli, XU Weisheng, YANG Hetong, LI Jishun, HE Chunyu. Effects of B418 on the yield of continuously cropped Rape (Brassica chinensis L.) and on the nitrogen-cycling and carbon-fixation gene cbbL in rhizosphere soils[J]. Shandong Science, 0, (): 1-.

参考文献

[1]王程,李玉双,侯永侠,等.生物炭对土壤中温室气体排放的影响及其机理研究进展 [J].北方园艺,2023(8):127-132.

[2]刘爽,姚佳妮,张钧杰,等.荒漠豆科灌丛根际土壤氨氧化和反硝化微生物功能基因丰度及群落多样性特征[J].草业学报, 2024, 33(5):15-127.

[3]郭辉,连延浩,赵志博,等.大豆和玉米茬口对冬小麦根际土壤细菌群落结构和功能的影响[J].河南农业科学,2024, ,53(7):79-87

[4] HU H W, CHEN D L, HE J Z. Microbial regulation of terrestrial nitrous oxide formation: Understanding the biological pathways for prediction of emission rates[J]. FEMS Microbiology Reviews, 2015, 39(5): 729-749. DOI:10.1093/femsre/fuv021.

[5] CUI Y X, ZHANG Y L, DUAN C J, et al. Ecoenzymatic stoichiometry reveals microbial phosphorus limitation decreases the nitrogen cycling potential of soils in semi-arid agricultural ecosystems[J]. Soil and Tillage Research, 2020, 197: 104463. DOI:10.1016/j.still.2019.104463.

[6] CHE R X, QIN J L, TAHMASBIAN I, et al. Litter amendment rather than phosphorus can dramatically change inorganic nitrogen pools in a degraded grassland soil by affecting nitrogen-cycling microbes[J]. Soil Biology and Biochemistry, 2018, 120: 145-152. DOI:10.1016/j.soilbio.2018.02.006.

[7]刘洋荧,王尚,厉舒祯,等.基于功能基因的微生物碳循环分子生态学研究进展[J].微生物学通报,2017,44(7) :1676-1689.

[8]袁红朝,秦红灵,刘守龙,等.固碳微生物分子生态学研究[J].中国农业科学, 2011, 44(14):2951-2958.

[9]肖红,刘玉玲,刘忠宽,等.氮磷添加对草甸草原土壤氮磷转化功能基因丰度的影响[J].生态学报,2023,43(1):313-326.

[10]唐存柳,靳泽文,姚光伟,等.生物质炭-沼液联合施用对调控氮循环功能基因促进氮素增效的影响[J].环境化学,2023, 42(8): 2843-2852.

[11]虞璐. 生物质炭对酸化土壤的改良效应及其对土壤硝化作用的影响[D].杭州: 浙江大学, 2019.

[12]刘发波,马笑,张芬,等.硝化抑制剂对我国蔬菜生产产量、氮肥利用率和氧化亚氮减排效应的影响: Meta分析[J]. 环境科学, 2022, 43(2): 5140-5148.

[13]童帅. 水稻、小麦内生固氮菌固氮酶nifH基因多样性及其活性研究[M].中国农业科学院, 2020.

[14]郝紫玉,邵亚旭,刘涛 ,等.东北农田黑土固碳微生物研究进展 [J].微生物学通报,2024, 51(6):1873-1886.

[15]刘敏敏,吴远征,扈进冬,等.越南伯克霍尔德氏菌B418的红色荧光蛋白标记及功能稳定性[J].山东科学,2023,36(1):51-57

[16] WEI Y L, YANG H, HU J D, et al.Trichoderma harzianuminoculation promotes sweet sorghum growth in the saline soil by modulating rhizosphere available nutrients and bacterial community[J]. Frontiers in Plant Science, 2023, 14: 1258131. DOI:10.3389/fpls.2023.1258131.

[17]董成,陈智勇,谢迎新,等.生物炭连续施用对农田土壤氮转化微生物及N2O排放的影响[J].中国农业科学,2020,53(19):4024-4034.

[18]刘茗,曹林桦,刘彩霞,等.亚热带 4 种典型森林植被土壤固碳细菌群落结构及数量特征[J].土壤学报, 2021, 58(4):1028–1039.

[19]储成,吴赵越,黄欠如,等.有机质提升对酸性红壤氮循环功能基因及功能微生物的影响[J].环境科学2020,41(5):2468-2475.

[20]刘领,马宜林,悦飞雪,等．生物炭对褐土旱地玉米季氮转化功能基因、丛枝菌根真菌及N2O释放的影响．生态学报, 2021, 41(7) :2803—2815.

[21]高晓红,刘洪波,赵志鑫,等.土壤微生物种类和数量与农作物产量和健康之间的关系[J].数字农业与智能农机,2024(1): 51-53.

[22]魏宏宇，李怡，彭帅英，等．胞外多糖促进胁迫条件下农作物生长的研究与展望［J］．江苏农业学报，2022，38( 4) : 1123-1134．

[23] ARTH I, FRENZEL P, CONRAD R. Denitrification coupleD to nitrification in the rhizosphere of rice[J]. Soil Biology and Biochemistry, 1998, 30(4): 509-515. DOI:10.1016/s0038-0717(97)00143-0.

[24] YANG Y D, HU Y G, WANG Z M, et al. Variations of the nirS-, nirK-, and nosZ-denitrifying bacterial communities in a northern Chinese soil as affected by different long-term irrigation regimes[J]. Environmental Science and Pollution Research, 2018, 25(14): 14057-14067. DOI:10.1007/s11356-018-1548-7.

[25] YIN C, FAN F L, SONG A L, et al. Denitrification potential under different fertilization regimes is closely coupled with changes in the denitrifying community in a black soil[J]. Applied Microbiology and Biotechnology, 2015, 99(13): 5719-5729. DOI:10.1007/s00253-015-6461-0.

[26]宋延静,马兰,李俊林,等.山东省盐渍化地区芦苇根际反硝化细菌群落结构研究 [J].山东农业科学,2021, 53(12):137-142.

[27] TABITA F R. Molecular and cellular regulation of autotrophic carbon dioxide fixation in microorganisms[J]. Microbiological Reviews, 1988, 52(2): 155-189. DOI:10.1128/mr.52.2.155-189.1988.

[28]张爽,刘涛,郝紫玉,等.东北黑土区农田土壤固氮微生物特征研究进展[J/OL].水土保持学报，https://doi.org/10.13870/j.cnki.stbcxb.2025.06.015

B418对连作小油菜产量及根际土壤氮循环基因和cbbL固碳基因的影响

Effects of B418 on the yield of continuously cropped Rape (Brassica chinensis L.) and on the nitrogen-cycling and carbon-fixation gene cbbL in rhizosphere soils

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

Metrics

本文评价

推荐阅读 0