B418对连作小油菜产量及根际土壤氮循环基因和cbbL固碳基因的影响

doi:10.3976/j.issn.1002-4026.2025076

山东科学 ›› 2026, Vol. 39 ›› Issue (5): 37-44.doi: 10.3976/j.issn.1002-4026.2025076

B418对连作小油菜产量及根际土壤氮循环基因和cbbL固碳基因的影响

王贻莲¹(), 扈进冬¹, 黄鼎立², 徐维生³, 杨合同¹, 李纪顺¹^,^*(), 何春雨⁴

¹ 齐鲁工业大学(山东省科学院) 生态研究所黄河流域轻工有机污染物低碳处置与资源化利用协同创新中心, 山东济南 250103
² 威海益丰农业科技有限公司, 山东威海 264200
³ 山东省沂水县诸葛镇林业站, 山东临沂 276422
⁴ 甘肃中医药大学药学院, 甘肃兰州 730000

收稿日期:2025-07-21 修回日期:2025-11-03 出版日期:2026-10-20 上线日期:2025-12-23
通信作者: *李纪顺(1971—),男,应用研究员,研究方向为农田退化土壤的修复。E-mail:sdyewu2@163.com
作者简介:王贻莲(1978—),女,硕士,副研究员,研究方向为设施蔬菜田退化土壤的生物修复。E-mail:yilianwang@163.com,Tel:13573791095
基金资助:
齐鲁工业大学(山东省科学院)科教产融合试点工程重大创新类项目(2025ZDZX18);齐鲁工业大学(山东省科学院)产学研协同创新基金项目(2020-CXY11);威海市政府-山东省科学院产学研协同创新基金项目(2020GC05)

Effects of B418 on the yield of continuously cropped rape(Brassica chinensis L.)and on the nitrogen-cycling and carbon-fixation gene cbbL in rhizosphere soils

WANG Yilian¹(), HU Jindong¹, HUANG Dingli², XU Weisheng³, YANG Hetong¹, LI Jishun¹^,^*(), HE Chunyu⁴

¹ Collaborative Innovation Center of Yellow River Basin for Low-Carbon Disposal and Resource Utilization of Organic Pollutants in Light Industry, Ecology Institute of Shandong Academy of Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China
² Weihai Yifeng Agricultural Science and Technology Co., Ltd., Weihai 264200, China
³ Forestry Station of Zhuge Town, Yishui County, Shandong Province, Linyi 276422, China
⁴ College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730000, China

Received:2025-07-21 Revised:2025-11-03 Published:2026-10-20 Online:2025-12-23

摘要/Abstract

摘要：

为探明越南伯克霍尔德氏菌B418缓解连作障碍土壤对小油菜生产的限制作用,采用盆栽试验,研究了外源添加B418对连作小油菜的出苗、存活及其根际土壤氮循环相关功能基因和固碳基因的影响,并探讨功能基因与产量的相关性,以期为连作蔬菜增产提供科学依据。结果表明:次生盐渍化轻度障碍土壤Ⅰ(pH 4.3,电导率1 361 μS/cm)和次生盐渍化重度障碍土壤Ⅱ(pH 4.6,电导率2 200 μS/cm)中,B418处理均能提高小油菜出苗率、存活率并增加产量,促使根际土壤固碳基因(cbbL)的绝度丰度增加。土壤Ⅰ中,B418处理固氮酶nifH和氨单加氧酶amoA基因丰度提高,氧化亚氮还原酶nosZ基因丰度降低;土壤Ⅱ中,B418处理nifH基因丰度提高,amoA、亚硝酸盐还原酶基因nirK、nirS和nosZ基因丰度降低,且处理间差异显著,表明外源添加B418可抑制连作小油菜土壤根际硝化作用和反硝化作用。相关性分析结果显示,产量与cbbL和nifH基因丰度呈正相关,与nirK基因丰度呈负相关,与amoA、nirS和nosZ基因丰度呈显著负相关;amoA与nosZ基因丰度呈极显著正相关,表明连作小油菜产量的提高与根际微生物的固碳、氮能力及抑制硝化作用和反硝化作用密切相关。

关键词: 越南伯克霍尔德氏菌B418, 连作障碍, 小油菜, 氮循环相关基因, 固碳基因丰度

Abstract:

To clarify how B418 alleviates the limitations imposed by continuous-cropping obstacle soils on rape production, a plot experiment was conducted to investigate the effects of exogenously applied B418 on the emergence, survival, and yield of rape, as well as on nitrogen transformation-related genes and carbon-fixation genes in the rhizosphere soil of continuously cropped rape (Brassica chinensis L.). Correlations between functional gene abundance and yield were also analyzed to provide a scientific basis for increasing the yield of continuously cropped vegetables. The results showed that in mildly secondary salinized obstacle soil I (pH 4.3, EC 1 361 μS/cm) and severely secondary salinized obstacle soil II (pH 4.6, EC 2 200 μS/cm), B418 significantly increased the emergence rate, survival rate, and yield of rape and increased the absolute abundance of the carbon-fixation gene cbbL in rhizosphere soil. In soil I, B418 treatment increased the abundance of the nitrogenase gene nifH and the ammonia monooxygenase gene amoA, but decreased the abundance of the nitrous oxide reductase gene nosZ. In soil II, B418 treatment increased the abundance of nifH while decreasing the abundance of amoA, the nitrite reductase genes nirK and nirS, and nosZ, with significant differences between treatments. These results indicate that exogenously added B418 can inhibit rhizosphere nitrification and denitrification in continuously cropped rape. Correlation analysis showed that yield was positively correlated with the abundance of cbbL and nifH, negatively correlated with nirK, and significantly negatively correlated with the abundance of amoA, nirS, and nosZ. The abundance of amoA was extremely significantly positively correlated with that of nosZ, indicating that yield improvement in continuously cropped rape is closely related to rhizosphere microbial carbon-fixation and nitrogen-cycling capacities, as well as to the inhibition of nitrification and denitrification.

Key words: Burkholderia vietnamiensis B418, continuous cropping obstacles, Brassica chinensis L., nitrogen-cycling genes, carbon-fixation gene abundance

中图分类号:

S636.9

王贻莲, 扈进冬, 黄鼎立, 徐维生, 杨合同, 李纪顺, 何春雨. B418对连作小油菜产量及根际土壤氮循环基因和cbbL固碳基因的影响[J]. 山东科学, 2026, 39(5): 37-44.

WANG Yilian, HU Jindong, HUANG Dingli, XU Weisheng, YANG Hetong, LI Jishun, HE Chunyu. Effects of B418 on the yield of continuously cropped rape(Brassica chinensis L.)and on the nitrogen-cycling and carbon-fixation gene cbbL in rhizosphere soils[J]. Shandong Science, 2026, 39(5): 37-44.

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土壤类型	w(N)/ (g·kg^-1)	w(P)/ (g·kg^-1)	w(K)/ (g·kg^-1)	w(有机质)/ (g·kg^-1)	w(有机碳)/ (g·kg^-1)	w(碱解氮)/ (mg·kg^-1)	w(有效磷)/ (mg·kg^-1)	w(速效钾)/ (mg·kg^-1)
土壤Ⅰ	0.85	0.60	22.2	14.70	8.5	237	70.4	143
土壤Ⅱ	0.80	0.58	21.5	15.1	8.8	261	56.8	160

土壤处理	出苗率/%	存活率/%	净菜重/(g·盆^-1)	增产率/%
土壤Ⅰ	87.50±9.57^a	85.00±2.45^a	5.20±1.15^ab	—
土壤Ⅰ+B418	95.00±5.77^a	90.00±8.17^a	6.57±1.18^a	26.35
土壤Ⅱ	60.00±2.65^b	23.33±5.77^b	1.75±0.95^b	—
土壤Ⅱ+B418	100.00±0.00^a	33.33±5.77^b	6.15±2.66^a	251.43

基因	nifH	amoA	nirK	nirS	nosZ	cbbL	产量
nifH	1	-0.060	0.44	-0.15	-0.15	-0.27	0.28
amoA		1	0.86	0.90	0.996^**	-0.37	-0.95^*
nirK			1	0.78	0.82	-0.57	-0.74
nirS				1	0.90	-0.69	-0.97^*
nosZ					1	-0.34	-0.97^*
cbbL						1	0.48
产量							1

B418对连作小油菜产量及根际土壤氮循环基因和cbbL固碳基因的影响

Effects of B418 on the yield of continuously cropped rape(Brassica chinensis L.)and on the nitrogen-cycling and carbon-fixation gene cbbL in rhizosphere soils

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