四种蔬菜土壤真菌群落组成与多样性

doi:10.3976/j.issn.1002-4026.2023.01.008

山东科学 ›› 2023, Vol. 36 ›› Issue (1): 58-65.doi: 10.3976/j.issn.1002-4026.2023.01.008

四种蔬菜土壤真菌群落组成与多样性

扈进冬(), 李红梅, 吴远征, 魏艳丽, 李纪顺^*()

齐鲁工业大学(山东省科学院) 山东省科学院生态研究所山东省应用微生物重点实验室,山东济南 250103

收稿日期:2022-04-01 出版日期:2023-02-20 发布日期:2023-02-08
通信作者: *李纪顺, 研究员,研究方向为生物制剂研发。E-mail:yewu2@sdas.org
作者简介:扈进冬(1977—),男, 高级工程师,研究方向为生物制剂研发。E-mail:hujd@sdas.org
基金资助:
山东省重大科技创新工程项目(2020CXGC010803);济南市高校院所创新团队项目(2020GXRC003);齐鲁工业大学(山东省科学院)校(院)地产学研协同创新基金(2020-CXY42);齐鲁工业大学(山东省科学院)校(院)地产学研协同创新基金(2020-CXY11)

Composition and diversity of fungal communities of four vegetable soils

HU Jindong(), LI Hongmei, WU Yuanzheng, WEI Yanli, LI Jishun^*()

Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University ofTechnology (Shandong Academy of Sciences), Jinan 250103, China

Received:2022-04-01 Online:2023-02-20 Published:2023-02-08

摘要/Abstract

摘要：

土壤真菌群落多样性是评价耕作土壤环境质量与健康的重要指标。以山东四种重要的蔬菜作物大葱、姜、草莓、黄瓜种植土壤为研究对象,采用qPCR和基于扩增子测序的高通量测序技术,结合土壤理化性质分析,研究了不同作物土壤真菌的群落组成及其多样性差异。结果表明:大葱土壤中以真菌ITS拷贝数为指标的总真菌丰度最低,且与草莓、黄瓜和姜土壤总真菌丰度具有显著差异(P<0.05);大葱和姜土壤真菌群落香农指数和操作分类单元指数高,多样性较丰富;黄瓜和草莓土壤真菌香农指数和操作分类单元指数低,多样性较差;土壤中子囊菌、担子菌、被孢菌门等相对丰度占比较高,其中在发病土壤中子囊菌门真菌相对丰度更高;土壤真菌类群受环境因子的影响,其中pH和有效磷对真菌群落构成的影响最大。这些结果为厘清不同真菌群落多样性与蔬菜土壤环境质量关系,为蔬菜土壤环境质量改善提供了理论依据。

关键词: 蔬菜土壤, 真菌群落, 多样性, 高通量测序, 冗余分析

Abstract:

The diversity of fungal communities is an important evaluation indicator for the environmental quality and health of cultivated soil. In this study, four vegetable (onion,ginger,strawberry,cucumber) soils were investigated to analyze the physical and chemical properties as well as the composition and diversity of fungal communities via Quantitative Polymerase Chain Reaction and high-throughput sequencing.The results showed that the fungal abundance inonion soil was the lowest and significantly different from that in the other three crops (strawberry,cucumber,ginger;P< 0.05).The Shannon index and OTUs of the onion and ginger soil were high, indicating higher fungal diversity,whereas the Shannon index and OTUs of cucumber and strawberry were low, indicating lower diversity. The relative abundances of Ascomycota,Basidiomycota, and Mortierellomycota were higher, with the relative abundance of Ascomycota being especially high in diseased soil. Soil fungal communities were affected by environmental factors, with pH and available phosphorus having the greatest influence.These results provided a theoretical basis for clarifying the relationship between the diversity of fungal communities and the quality of vegetable soil,which helped in improving the ecological quality of vegetable soil.

Key words: vegetable soil, fungal communities, diversity, high-throughput sequencing, redundancy analysis

中图分类号:

S157.1

扈进冬, 李红梅, 吴远征, 魏艳丽, 李纪顺. 四种蔬菜土壤真菌群落组成与多样性[J]. 山东科学, 2023, 36(1): 58-65.

HU Jindong, LI Hongmei, WU Yuanzheng, WEI Yanli, LI Jishun. Composition and diversity of fungal communities of four vegetable soils[J]. Shandong Science, 2023, 36(1): 58-65.

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参考文献 17

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作物类型	全氮	有效磷	速效钾	碱解氮	pH	电导率
黄瓜	2 572.56±1 314.98a	403.10±44.32a	359.10±157.21a	213.27±102.86a	7.68±0.41ab	463.84±236.19a
草莓	2 572.63±523.36b	153.55±53.41b	262.60±81.42b	127.64±52.97b	7.49±0.24b	244.87±135.10b
大葱	1 061.67±552.51b	64.65±15.54c	212.71±47.33b	61.86±21.44c	6.27±0.44c	161.33±216.80b
姜	1 243.53±416.24b	185.60±62.23b	206.90±68.87b	127.26±31.10b	7.85±0.24c	140.47±45.98b

环境因子	RDA1	RDA2	r²	P
全氮	0.999 9	-0.013 9	0.203 9	0.024 9
有效磷	0.946 4	-0.322 9	0.367 3	0.000 5
速效钾	0.821 8	-0.569 7	0.311 8	0.002 9
碱解氮	0.999 2	0.038 9	0.191 2	0.026 4
pH	0.953 6	0.300 8	0.438 7	0.000 5
电导率	0.590 5	-0.806 9	0.231 4	0.009 9

四种蔬菜土壤真菌群落组成与多样性

Composition and diversity of fungal communities of four vegetable soils

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