土壤杆菌固氮、溶磷活性及对植物的促生作用

doi:10.3976/j.issn.1002-4026.2017.04.005

山东科学 ›› 2017, Vol. 30 ›› Issue (4): 26-30.doi: 10.3976/j.issn.1002-4026.2017.04.005

土壤杆菌固氮、溶磷活性及对植物的促生作用

魏艳丽¹，Maarten RYDER ²，李纪顺¹，李玲¹，杨玉忠¹，杨合同^1,3*

1. 山东省科学院生态研究所，山东省应用微生物重点实验室，山东济南 250014； 2. 澳大利亚阿德莱德大学农业食品葡萄酒学院，阿德莱德 5064；3. 山东省黄河三角洲可持续发展研究院，山东东营 257000

收稿日期:2017-05-11 出版日期:2017-08-20 发布日期:2017-08-20
通信作者: 杨合同，研究员，黄河三角洲学者特聘专家，研究方向为农业病害生物防治。E-mail: yanght@sdas.org E-mail:yanght@sdas.org
作者简介:魏艳丽（1978—），女，高级工程师，研究方向为农业微生物。E-mail: yanli_wei@163.com
基金资助:
山东省重点研发计划（2016GNC113010）；2017年度山东省引进国外智力成果示范推广项目；黄河三角洲学者工程专项

Nitrogen fixation, phosphate solubilizing activity and plant growth promotion of Agrobacterium

WEI Yan-li¹, Maarten RYDER ², LI Ji-shun¹, LI Ling¹, YANG Yu-zhong¹, YANG He-tong^1,3*

1. Ecology Institute of Shandong Academy of Sciences, Shandong Provincial Key Laboratory of Applied Microbiology, Jinan 250014, China; 2. School of Agriculture, Food and Wine, University of Adelaide, Adelaide 5064, Australia; 3.Research Center of Sustainable Development in the Yellow River Delta，Dongying 257000, China

Received:2017-05-11 Online:2017-08-20 Published:2017-08-20

摘要/Abstract

摘要：

通过选择性培养基，定性和定量检测了26株土壤杆菌的固氮和溶磷活性，通过盆栽检测了澳洲生防菌株K1026对黄瓜和小麦的促生长作用。结果显示，包括致病性和无致病性的26株土壤杆菌均可在无氮培养基上正常生长；其中19株具有溶磷活性， K1026对磷酸钙液体培养基的溶磷量为109.11 μg/mL；对黄瓜幼苗根系生长具有显著促进作用，但对小麦生长没有明显影响。

关键词: Agrobacterium rhizogenes K1026, 促生作用, 固氮, 土壤杆菌, 溶磷

Abstract:

Through selective medium, the function of fixing nitrogen and dissolving phosphorus of 26 Agrobacterium strains were studied by both qualitative and quantitative analysis methods. Meanwhile, growthpromoting effect of Australian biocontrol strain K1026 on cucumber and wheat was measured by pot experiment. The results showed that all the strains regardless of pathogenicity or nonpathogenicity could grow normally on nitrogenfree medium. 19 of the 26 strains had the ability to dissolve phosphorus, the Phosphate released by K1026 in Pikovskaya broth medium was up to 109.11 μg/mL , having the strongest activities among all stains. The pot experiments showed that K1026 had obvious growthpromoting effect on the cucumber seedlings, and the root length and the dry weight of the root biomass was increased by 25.49%, 62.96%, respectively, compared with the control. However, K1026 had no obvious effect on the growthpromotion of wheat.

Key words: Agrobacterium rhizogenes K1026, phosphate-solubilisation, growth promotion effect, Agrobacterium tumefaciens, nitrogen fixation

中图分类号:

S436.629

魏艳丽，Maarten RYDER,李纪顺，李玲，杨玉忠，杨合同. 土壤杆菌固氮、溶磷活性及对植物的促生作用[J]. 山东科学, 2017, 30(4): 26-30.

WEI Yan-li, Maarten RYDER,LI Ji-shun, LI Ling, YANG Yu-zhong, YANG He-tong. Nitrogen fixation, phosphate solubilizing activity and plant growth promotion of Agrobacterium[J]. SHANDONG SCIENCE, 2017, 30(4): 26-30.

参考文献

［1］CLARE B G, KERR A, JONES D A. Characteristics of the nopaline catabolic plasmid in Agrobacterium strains K84 and K1026 used for biological control of crown gall disease ［J］.Plasmid, 1990, 23(2): 126137.
［2］KERR A. Biological control of crown gall ［J］. Australasian Plant Pathology, 2016, 45: 1518.
［3］PUAWSKA J, WARABIEDA W, ISMAIL E. Identification and characterization of bacteria isolated from crown galls on stone fruits in Poland ［J］. Plant Pathology, 2016, 65: 10341043.
［4］KUZMMANOVIc'N, PROKIc'A, IVANOVIc'M, et al. Genetic diversity of tumorigenic bacteria associated with crown gall disease of raspberry in Serbia ［J］. European Journal Plant Pathology, 2015, 142: 701713.
［5］李纪顺，陈凯，杨合同，等.伯克霍尔德氏菌B418 的生物学特性［C］//中国植物病理学会2004年学术年会论文集. 北京:中国农业科学技术出版社, 2004:432437.
［6］PIKOVSKAYA R I. Mobilization of phosphorus in soil in connection with vital activity of some microbial species［J］. Microbiologiya, 1948, 17:362370.
［7］LI J F, ZHANG S Q, SHI S L, et al. Mutational approach for N2fixing and Psolubilizing mutant strains of Klebsiella pneumoniae RSN19 by microwave mutagenesis［J］. World J Microbiol Biotechnol, 2011,27(6): 14811489.
［8］KUZMMANOVIc'N, IVANOVIc'M, PROKIc'A, et al. Characterization and phylogenetic diversity of Agrobacterium vitis from Serbia based on sequence analysis of 16S23S rRNA internal transcribed spacer (ITS) region［J］. European Journal of Plant Pathology, 2014,140(4): 757768.
［9］LASSALLE F, CAMPILLO T, VIAL L, et al. Genomic species are ecological species as revealed by comparative genomics in Agrobacterium tumefaciens［J］. Genome Biology and Evolution, 2011,3: 762781.
［10］王丽，张静娟.土壤杆菌固氮生理特性研究［J］.微生物学报,1994,34(5):385392.
［11］林英，司春灿，韩文华，等.解磷微生物研究进展［J］. 江西农业学报，2017,29( 2)：99103.
［12］RODRGUEZ H, FRAGA R. Phosphate solubilizing bacteria and their role in plant growth promotion ［J］. Biotechnology Advances, 1999，17 (4/5) :319339.
［13］HAO Y, CHARLES T C, GLICK B R. ACC deaminase activity in avirulent Agrobacterium tumefaciens D3［J］.Can J Microbiol, 2011, 57(4), 278286.

[1]	吴晓青，赵忠娟，李哲，扈进冬，赵晓燕，王贻莲，黄玉杰，李纪顺，杨合同. 施加木霉可湿性粉剂对冬小麦田间生长的影响[J]. 山东科学, 2015, 28(6): 35-42.
[2]	李善梅, 李纪顺, 徐维生, 陈凯, 杨合同. 生防菌剂YKT41及B418防治茄子根结线虫病的田间试验[J]. J4, 2011, 24(5): 10-13.

土壤杆菌固氮、溶磷活性及对植物的促生作用

Nitrogen fixation, phosphate solubilizing activity and plant growth promotion of Agrobacterium

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

Metrics

本文评价

推荐阅读 0