关键词: 环磷酸腺苷, PB实验, BB实验, 微波-超声波提取

Abstract: To improve the extraction rate of cAMP from Ziziphus jujube, we identified the significant factors affecting cAMP extraction ratio, microwave time, solidliquid ratio, ultrasonic time and power, with PlackettBurman (PB) experiment and jujube as a raw material. We further acquired the most obvious influential factor of ultrasonic time, the second significant factor of solidliquid ratio, and the least significant factors of microwave time and ultrasonic power in designated scopes, with BoxBehnken (BB) experiment and response surface analysis. Its optimal extraction conditions are microwave power of 440 W, microwave time of 64 s, solidliquid ratio of 1:8, ultrasonic time of 21min, ultrasonic power of 300 W, and ultrasonic temperature of 20 ℃. Extraction rate of cAMP is 90.83% under these conditions. The research result can provide a better natural active substance for pharmaceutical and food industries, and a reference for the further processing and industrialized applications of Ziziphus jujube and other relative products.

Key words: BB experiment, PB experiment, cAMP, microwaveultrasonic extraction