超高压辅助浊点萃取法提取富集莲子心总生物碱

doi:10.3976/j.issn.1002-4026.2013.03.004

J4 ›› 2013, Vol. 26 ›› Issue (3): 15-20.doi: 10.3976/j.issn.1002-4026.2013.03.004

• 中药与天然活性产物专栏 • 上一篇下一篇

超高压辅助浊点萃取法提取富集莲子心总生物碱

朱荣华，王晓，刘建华，张树芹，刘峰

1. 山东农业大学化学与材料科学学院，山东泰安 271018； 2. 山东省分析测试中心，山东济南 250014

收稿日期:2012-10-21 出版日期:2013-06-20 发布日期:2013-06-20
通信作者: 刘峰，男，博士，研究方向为天然产物。 E-mail:liufeng8109@163.com
作者简介:朱荣华(1987-)，女，硕士研究生，研究方向为天然产物。Email：rhzhu6@163.com
基金资助:
山东省科技发展计划(2011SJGZ19)；山东省分析测试中心科技发展计划项目

Extraction and enrichment of total alkaloids of Plumula Nelumbinis by ultrahigh pressureassisted micellar extraction combined with cloudpoint extraction

ZHU Rong-Hua, WANG Xiao, LIU Jian-Hua, ZHANG Shu-Qin, LIU Feng

1. School of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, China; 2. Shandong Analysis and Test Center, Jinan 250014, China

Received:2012-10-21 Online:2013-06-20 Published:2013-06-20

摘要/Abstract

摘要：

通过表面活性剂类型、浓度、提取压力、时间、料液比等单因素实验确定了超高压辅助胶束提取的工艺条件，并通过加入NaCl和加热引发体系浊点，诱导双水相分离，实现了莲子心生物碱的富集。结果表明，最佳提取工艺为质量分数5%的OP10为溶剂，提取压力100 MPa，提取时间1 min，料液比1:20(g/mL)，莲子心生物碱的提取率可达到103.6 mg/g。提取液中加入NaCl使其质量分数达到15%，在50℃条件下平衡10 min，两相分离，离心后莲子心生物碱的萃取回收率为70.73%，浓缩系数为8.6。此方法快速高效、环境友好。

关键词: 莲子心, 生物碱, 超高压辅助胶束提取, 浊点萃取

Abstract:

We determined the technical conditions of ultrahigh pressureassisted micellar extraction by such singleelement experiment as surface active agent type, concentration, extraction pressure, time and the ratio of solid and liquid to construct the extraction and enrichment process of total alkaloids of Plumula Nelumbinis. We implemented the enrichment of the alkaloids of Plumula nelumbinis by NaCl addition, cloud point extraction and double water phase separation. The optimal extraction conditions are 5%(w/w) OP10 solvent, extraction pressure of 100 MPa, extraction time of 1 min and solid/liquid ratio of 1:20 (g/mL). The optimal extraction yield of total alkaloids is 103.6 mg/g. Its recovery rate is 70.73% and the preconcentration factor (CF) is 8.6 under the conditions of 15% NaCl addition, stability of 10 min at 50 ℃ and twophase separation. Results show that the extraction process is rapid and benefit for environment.

Key words: Plumula Nelumbinis, alkaloids, ultrahigh pressureassisted micellar extraction, cloudpoint extraction

中图分类号:

R284.2

朱荣华，王晓，刘建华，张树芹，刘峰. 超高压辅助浊点萃取法提取富集莲子心总生物碱[J]. J4, 2013, 26(3): 15-20.

ZHU Rong-Hua, WANG Xiao, LIU Jian-Hua, ZHANG Shu-Qin, LIU Feng. Extraction and enrichment of total alkaloids of Plumula Nelumbinis by ultrahigh pressureassisted micellar extraction combined with cloudpoint extraction[J]. J4, 2013, 26(3): 15-20.

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超高压辅助浊点萃取法提取富集莲子心总生物碱

Extraction and enrichment of total alkaloids of Plumula Nelumbinis by ultrahigh pressureassisted micellar extraction combined with cloudpoint extraction

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