山东科学 ›› 2011, Vol. 24 ›› Issue (6): 56-61.doi: 10.3976/j.issn.1002-4026.2011.06.015

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非致病性双核丝核菌BNR-1对小麦防御酶系的影响

 周红姿1, 陈凯1, 赵晓燕1, 李纪顺1, 张新建1, 杨合同1,2*   

  1. 1.山东省科学院中日友好生物技术研究中心,山东省应用微生物重点实验室,山东 济南 250014; 2.山东省科学院生物研究所,山东 济南 250014
  • 收稿日期:2011-07-18 出版日期:2011-12-20 发布日期:2011-12-20
  • 通信作者: 杨合同(1966-),男,研究员,研究方向为农业微生物防治. E-mail:yanght@keylab.net
  • 作者简介:周红姿(1976-),女,助理研究员,硕士,研究方向为农业微生物防治,Email:trichodermazh1976@yahoo.com.cn
  • 基金资助:

    国际科技合作项目(2009DFA32340)

Impact of nonpathogenic binucleate Rhizoctonia spp. BNR-1 on defense enzyme activities of wheat

 ZHOU Hong-Zi1, CHEN Kai1, ZHAO Xiao-Yan1, LI Ji-Shun1, ZHANG Xin-Jian1, YANG He-Tong1,2   

  1. 1.Shandong Provincial Key Laboratory of Applied Microbiology, Biotechnology Center, Shandong Academy of Sciences,Jinan 250014, China; 2.Biology Research Institute, Shandong Academy of Sciences, Jinan 250014, China
  • Received:2011-07-18 Online:2011-12-20 Published:2011-12-20

摘要:

    用非致病性双核丝核菌(Nonpathogenic Binucleate Rhizoctonia species)菌株BNR-1接种处理小麦植株根际,测定一定生长期内小麦防御酶活性变化,结果表明经过BNR-1处理的小麦样品,其防御酶过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸转氨酶(PAL)、几丁质酶和β-1,3-葡聚糖酶活性明显提高,而且经过BNR1处理的小麦样品纹枯病(wheat sharpeye spot)发病率明显降低。

关键词: 非致病性双核丝核菌, 过氧化物酶, 多酚氧化酶, 苯丙氨酸转氨酶, 几丁质酶, β-1,3-葡聚糖酶

Abstract:

       We employed the strain, BNR-1, of Nonpathogenic Binucleate Rhizoctonia species to inoculate the roots of wheat and to determine the change of wheat defense enzyme activity in a certain vegetative period. Results show that the activities of peroxidase(POD), polyphenoloxidase(PPO), phenylalanine ammonialyase(PAL), chitinase and β-1,3-glucanase of wheat samples are significantly enhanced after they are processed by BNR-1. Moreover, their sharp eyespot incidence is dramatically decreased after they are processed by BNR-1.

Key words: Nonpathogenic Binucleate Rhizoctonia species, peroxidase, polyphenoloxidase, phenylalanine ammonialyase, chitinase, β-1,3-glucanase

中图分类号: 

  • S476+.9