基于成分-抗氧化关联的洋蓟花苞不同溶剂萃取物差异分析

doi:10.3976/j.issn.1002-4026.20230039

摘要/Abstract

摘要：

基于成分-抗氧化关联模式探究洋蓟花苞不同溶剂萃取物中活性成分质量分数及抗氧化功能差异。分别采用水、70%甲醇、70%乙醇和70%丙酮萃取洋蓟花苞,通过对比各种萃取物中总多酚、总黄酮的质量分数,并利用1,1-二苯基-2-三硝基苯肼(DPPH)、2,2'-联氮-二(3-乙基-苯并噻唑啉-6-磺酸)二铵盐(ABTS)阳离子清除能力和总还原能力评估其抗氧化活性,综合分析洋蓟花苞不同萃取物主要活性成分质量分数与抗氧化能力之间的相关性。结果表明:70%乙醇洋蓟花苞萃取物中总多酚、总黄酮质量分数最大,为(9.14±0.12) mg/g和(13.46±0.42) mg/g;70%甲醇萃取物综合抗氧化能力最强,清除DPPH、ABTS⁺的IC₅₀值分别为0.43 mg/mL和0.10 mg/mL,其萃取物还原力为0.5时对应的萃取物质量浓度(A_0.5)为6.42 mg/mL。相关性分析结果显示,总多酚和总黄酮质量分数与DPPH、ABTS⁺清除力呈极显著相关(P<0.01)。70%甲醇洋蓟花苞萃取物中含有丰富的多酚、黄酮类成分,抗氧化能力最强,该研究可为进一步开发利用洋蓟资源提供理论依据。

关键词: 洋蓟花苞, 不同溶剂, 关联分析, 活性成分, 抗氧化

Abstract:

Based on the component-antioxidant correlation model, the differences in the content of active components and the antioxidant function of different solvent extracts from artichoke bud were explored. Artichoke bud was extracted with water, 70% methanol, 70% ethanol, and 70% acetone. Total polyphenol and flavonoid contents were compared in the various extracts. The 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation scavenging capacity and the total reduction capacity were used to evaluate their antioxidant activity. The correlation between the main active ingredients and antioxidant capacity of different extracts from artichoke bud was comprehensively analyzed. The results showed that the levels of total polyphenols and total flavonoids in 70% ethanol extract were the highest, which were (9.14±0.12) mg/g and (13.46±0.42) mg/g, respectively. The extract with 70% methanol has the strongest antioxidant capacity. The IC₅₀ values of scavenging DPPH and ABTS⁺ were 0.43 mg/mL and 0.10 mg/mL, respectively. Additionally, when the reducing capacity was 0.5, the mass concentration (A_0.5) of the extract was 6.42 mg/mL. The results of correlation analysis showed that the level of total polyphenols and total flavonoids were significantly correlated with the DPPH and ABTS⁺ scavenging capacity (P<0.01). The extract with 70% methanol was rich in polyphenols and flavonoids and possessed the strongest antioxidant capacity, which can provide a certain theoretical basis for further development and utilization of artichoke resources.

Key words: artichoke bud, different solvents, correlation analysis, active ingredients, antioxidants

中图分类号:

R284.1

刘兰玲, 徐中利, 陈炯朝, 颜培正, 赵东升, 李佳. 基于成分-抗氧化关联的洋蓟花苞不同溶剂萃取物差异分析[J]. 山东科学, 2024, 37(1): 17-23.

LIU Lanling, XU Zhongli, CHEN Jiongchao, YAN Peizheng, ZHAO Dongsheng, LI Jia. Differential analysis of different solvent extracts from artichoke bud based on component-antioxidant correlation[J]. Shandong Science, 2024, 37(1): 17-23.

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样品	总多酚质量分数/(mg·g^-1)	总黄酮质量分数/(mg·g^-1)
水萃取物	7.89±0.06^c	10.35±0.43^c
70%甲醇萃取物	9.07±0.08^ab	12.55±0.31^b
70%乙醇萃取物	9.14±0.12^a	13.46±0.42^a
70%丙酮萃取物	8.50±0.20^b	12.16±0.19^b

	总多酚	总黄酮	DPPH	ABTS⁺	还原力
总多酚	1.000	0.847^**	0.931^**	0.855^**	-0.193
总黄酮		1.000	0.908^**	0.742^**	0.211
DPPH			1.000	0.854^**	0.136
ABTS⁺				1.000	0.008
还原力					1.000