基于液相色谱-质谱联用法建立盐酸坦洛新缓释片比格犬体内含量检测方法

doi:10.3976/j.issn.1002-4026.2021.02.005

山东科学 ›› 2021, Vol. 34 ›› Issue (2): 34-41.doi: 10.3976/j.issn.1002-4026.2021.02.005

基于液相色谱-质谱联用法建立盐酸坦洛新缓释片比格犬体内含量检测方法

郜琪臻^1，2，刘芳瑜³，丁平田³

1. 中国医科大学附属第一医院药学部，辽宁沈阳 110001；2. 中国医科大学药学院，辽宁沈阳 110122；3. 沈阳药科大学药学院，辽宁沈阳 110016

收稿日期:2020-06-03 出版日期:2021-04-13 发布日期:2021-04-13
作者简介:郜琪臻（1968—），男，副主任药师，研究方向为药物临床检测。E-mail:gaoqizhensy@163.com
基金资助:
国家自然科学基金青年基金（81703427）；辽宁省科学技术计划（2014226033）

A method for detecting the content of tamsulosin hydrochloride sustained-release tablets in Beagle dogs based on liquid chromatography-mass spectrometry

GAO Qi-zhen^1,2, LIU Fang-yu³, DING Ping-tian³

1. Department of Pharmacy, the First Affiliated Hospital of China Medical University, Shenyang 110001, China 2. School of Pharmaceutical Science, China Medical University, Shenyang 110122,China;3. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China

Received:2020-06-03 Online:2021-04-13 Published:2021-04-13

摘要/Abstract

摘要： 建立了一种基于液相色谱-质谱联用法的市售盐酸坦洛新缓释片的动物体内含量变化检测方法，以比格犬为实验动物绘制药物药动学曲线，并验证方法的可靠性。色谱柱为Zorbax Extend-C18柱（150 mm×4.6 mm，5 μm），保护柱为C18柱（4.0 mm × 3.0 mm），流动相为甲醇-水-甲酸（V（甲醇）:V（水）:V（甲酸）=85:14:1），流速为0.6 mL/min，柱温为30 ℃，进样量为20 μL。采用ESI离子源正离子多反应监测（MRM）模式选择离子对409.3→228.2、256.3→167.1进行测定。结果表明，盐酸坦洛新在0.1～10.0 ng/mL浓度范围内呈良好线性相关性，精密度为7.1%，提取回收率为92%，盐酸坦洛新缓释片的体内含量变化被准确描述。该方法灵敏度高、专属性好、精密度高，可用于简单快捷地检测市售盐酸坦洛新缓释片的动物体内含量变化。

关键词: 盐酸坦洛新缓释片, 液相色谱-质谱联用, 比格犬, 体内含量, 药动学

Abstract: In this study, we developed a method based on liquid chromatography-mass spectrometry to detect content changes in animals administered commercially available tamsulosin hydrochloride sustained-release tablets. Beagle dogs were used as the experimental animals to construct drug pharmacokinetic curves as well as to verify the reliability of the method. The column used was the Zorbax Extend-C18 column (150 mm×4.6 mm, 5 μm). C18 (4.0 mm×3.0 mm) was used as the guard column, and the mobile phase was methanol-water-formic acid (V（methanol）:V(water):V(formic)=85:14:1). The flow rate was 0.6 mL/min, column temperature was 30 ℃, and injection volume was 20 μL. The ESI ion source positive ion multiple reaction monitoring mode was used to select ion pairs 409.3→228.2 and 256.3→167.1 for determination. The results showed that tamsulosin hydrochloride has a good linear correlation in the concentration range of 0.1~10.0 ng/mL. The precision was 7.1%, and the recovery rate of extraction was 92%. The content changes of tamsulosin hydrochloride sustained-release tablets in Beagle dogs were accurately described. The above method has high sensitivity, good specificity, and high precision and can quickly and easily detect the content changes in animals administered commercially available tamsulosin hydrochloride sustained-release tablets.

Key words: tamsulosin hydrochloride sustained-release tablets')">

tamsulosin hydrochloride sustained-release tablets, liquid chromatography-mass spectrometry, Beagle dog, body content; pharmacokinetics

中图分类号:

R917

郜琪臻, 刘芳瑜, 丁平田. 基于液相色谱-质谱联用法建立盐酸坦洛新缓释片比格犬体内含量检测方法[J]. 山东科学, 2021, 34(2): 34-41.

GAO Qi-zhen, LIU Fang-yu, DING Ping-tian. A method for detecting the content of tamsulosin hydrochloride sustained-release tablets in Beagle dogs based on liquid chromatography-mass spectrometry[J]. Shandong Science, 2021, 34(2): 34-41.

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基于液相色谱-质谱联用法建立盐酸坦洛新缓释片比格犬体内含量检测方法

A method for detecting the content of tamsulosin hydrochloride sustained-release tablets in Beagle dogs based on liquid chromatography-mass spectrometry

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